文献类型：期刊文献

英文题名：Pendant small functional groups on poly(E-caprolactone) substrate modulate adhesion, proliferation and differentiation of human mesenchymal stem cells

作者：Chen, Min[1];Zhang, Yi[2];Zhou, Yan[1];Zhang, Yan[2];Lang, Meidong[2];Ye, Zhaoyang[1];Tan, Wen-Song[1]

机构：[1]E China Univ Sci & Technol, Sch Bioengn, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]E China Univ Sci & Technol, Sch Mat Sci & Engn, Minist Educ, Key Lab Ultrafine Mat, Shanghai 200237, Peoples R China

年份：2015

卷号：134

起止页码：322

外文期刊名：COLLOIDS AND SURFACES B-BIOINTERFACES

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000362142000040)】；

基金：This work was financially supported by National Natural Science Foundation of China (31000424, 31170951), Shanghai Pujiang Program (10PJ1402200), and Specialized Research Fund for the Doctoral Program of Higher Education (20100074120009).

语种：英文

外文关键词：Poly(epsilon-caprolactone); Small functional groups; Mesenchymal stem cells; Cell fate; Tissue engineering

摘要：Probing stem cell-biomaterial interactions is of great significance in both gaining profound understanding of stem cell biology and advancing tissue regeneration. In the present work, we developed a series of poly(E-caprolactone) (PCL) films bearing distinct pendant small functional groups to study the effects of biomaterial substrate chemistry on stem cell behaviors. PCL films, bearing hydroxyl (-OH), methyl (-CH3), carboxyl (-COOH) and amino (-NH2), demonstrated varied surface properties, such as wettability, serum protein adsorption and surface topographical feature. In comparison with pristine PCL film, the adhesion of hMSCs on PCL-COOH, PCL-OH and PCL-C=O films was significantly promoted and cells slightly outgrew on PCL-NH2 and PCL-COOH films. Most importantly, the tri-lineage differentiation of hMSCs varied on this series of PCL films, with the best osteogenesis achieved on PCL-NH2 film, PCL and PCL-CH3 films supporting the superior adipogenic differentiation and PCL-CH3 film being the most favorable one for chondrogenesis. This study highlights the critical roles of surface chemistry in modulating the fates of MSCs and potentially provides a practical guidance in developing instructive tissue engineering scaffolds. (C) 2015 Elsevier B.V. All rights reserved.