文献类型：期刊文献

英文题名：Rapid probing of the reactivity of P450 monooxygenases from the CYP116B subfamily using a substrate-based method

作者：Li, Ren-Jie[1];Xu, Jian-He[1];Yin, Yue-Cai[1];Wirth, Nicolas[1];Ren, Jiang-Meng[2];Zeng, Bu-Bing[2];Yu, Hui-Lei[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Shanghai Key Lab New Drug Design, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2016

卷号：40

期号：10

起止页码：8928

外文期刊名：NEW JOURNAL OF CHEMISTRY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000384754300091)】；

基金：This work was financially supported by the National Natural Science Foundation of China (No. 21672063 & 21536004), the Ministry of Science and Technology, P. R. China (No. 2011AA02A210) and Fundamental Research Funds for the Central Universities.

语种：英文

摘要：Developing a detailed understanding of the reactivity of self-sufficient Type IV P450 monooxygenases, four types of O-methylated substrates were designed as probes, including monoterpenes, cycloalkanes, aromatic compounds and steroids, and the efficiency of their oxyfunction was determined using a colorimetric assay which was based on the reaction between the enzymatic demethylation product, formaldehyde, and Purpald dye. The activity-based fingerprints of new P450(RpMO), P450(ArMO) and P450(CtMO) (CYP116B members) indicated that CYP116B P450s preferentially oxidize substrates with aromatic components. Moreover, the hydroxylated products were detected based on the preference results. This rapid and efficient strategy, when coupled with GCMS, enables the exploration of the reactivity of other CYP116B members.