文献类型：期刊文献

英文题名：Metaproteomics: A strategy to study the taxonomy and functionality of the gut microbiota

作者：Wang, Yuqiu[1,2,3,4];Zhou, Yanting[1,2];Xiao, Xiao[3];Zheng, Jing[3];Zhou, Hu[1,2]

机构：[1]Chinese Acad Sci, Shanghai Inst Mat Med, Dept Analyt Chem, Shanghai 201203, Peoples R China;[2]Chinese Acad Sci, Shanghai Inst Mat Med, CAS Key Lab Receptor Res, Shanghai 201203, Peoples R China;[3]East China Univ Sci & Technol, Sch Pharm, Shanghai 200237, Peoples R China;[4]East China Univ Sci & Technol, Sch Bioengn, Shanghai 200237, Peoples R China

年份：2020

卷号：219

外文期刊名：JOURNAL OF PROTEOMICS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000527378000005)】；

基金：This study was supported by Grant No. 2017YFC1700200 of the National Key Research and Development Program from the Ministry of Science and Technology of China, and the Nova Development Program of the Shanghai Institute of Materia Medica to Yanting Zhou.

语种：英文

外文关键词：Metaproteomics; Gut microbiota; Metagenomics; Metatranscriptomics; Metabolomics; Multi-omics

摘要：The gut microbiota is the largest and most complex microbial community in the human body. Host-gut microbiota interactions have significant implications on health and disease. The development of genome-sequencing technologies, especially the application of next-generation sequencing (NGS), has accelerated the study of the gut microbiota. Most gut microbiota studies rely on 16S rRNA sequencing, metagenomics, and metatranscriptomics, but metaproteomics, based on mass spectrometry (MS), provides functional information on the signaling and metabolic pathways in the gut microbiota. This review is intended to introduce different research methods to study the gut microbiota, with a specific focus on the current progress and application of metaproteomics. Significance: The gut microbiota plays a key role in human health and disease. In this review, different research methods are described and compared in the field of the gut microbiota. Among these research methods, metaproteomics reveals the taxonomy and functionality of the gut microbiota, especially the functional pathways associated with diseases. Thus, the current progress and application of metaproteomics are summarized, in order to enhance a comprehensive depiction of metaproteomics.