文献类型：期刊文献

中文题名：Two-step Enzymatic Synthesis of Guanine Arabinoside

英文题名：Two-step Enzymatic Synthesis of Guanine Arabinoside

作者：Wei Xiaokun[1,2,3];Ding Qingbao[1];Ou Lin[1];Zhang Lu[3];Wang Changlu[2]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Tianjin Univ Sci & Technol, Tianjin 300222, Peoples R China;[3]Shanghai Weiping Biosci & Biotechnol Co Ltd, Shanghai 200237, Peoples R China

年份：2008

卷号：16

期号：6

起止页码：934

中文期刊名：Chinese Journal of Chemical Engineering

外文期刊名：CHINESE JOURNAL OF CHEMICAL ENGINEERING

收录：CSTPCD;;EI(收录号：20090211851312);Scopus;WOS:【SCI-EXPANDED(收录号:WOS:000262274100019)】；CSCD:【CSCD2011_2012】；

基金：Supported by the Innovation Fund for Technology Based Firms from Ministry of Science and Technology of China (07C26213101283).

语种：英文

中文关键词：guanine arabinoside; 2;6-diaminopurine arabinoside; purine nucleoside phosphorylase; pyrimidine nucleoside phosphorylase; adenylate deaminase; Enterobacter aerogenes; Aspergillus oryzae

外文关键词：guanine arabinoside; 2,6-diaminopurine arabinoside; purine nucleoside phosphorylase; pyrimidine nucleoside phosphorylase; adenylate deaminase; Enterobacter aerogenes; Aspergillus oryzae

摘要：The chemical synthesis of Guanine arabinoside （ara-G） is extremely complex, time-consuming, and seriously polluted. A two-step enzymatic synthesis process was developed to acquire ara-G easily. 2,6-Diaminopurine arabinoside （ara-DA） was first synthesized with purine nucleoside phosphorylase and pyrimidine nucleoside phosphorylase produced by Enterobacter aerogenes DGW-07. The conversion yield of ara-DA could reach above 90% when the reaction liquid contained 30 mmol·L^-1 uracil arabinoside as arabinose donor, 10 mmol·L^- 1 2,6-diaminopurine as arabinose acceptor in pH 7.0 20 mmol·L^-1 phosphate buffer, and reacted at 60℃ for 48h. Then, ara-DA was effectively transformed into ara-G with adenylate deaminase produced by Aspergillus oryzae DAW-01. The total process had no complex separation and purification.
The chemical synthesis of Guanine arabinoside (ara-G) is extremely complex, time-consuming. and seriously polluted. A two-step enzymatic synthesis process was developed to acquire ara-G easily. 2,6-Diaminopurine arabinoside (ara-DA) was first synthesized with purine nucleoside phosphorylase and pyrimidine nucleoside phosphorylase produced by Enterobacter aerogenes DGW-07. The conversion yield of ara-DA could reach above 90% when the reaction liquid contained 30 mmol.L-1 uracil arabinoside its arabinose donor, 10 mmol.L-1 2,6-diaminopurine as arabinose acceptor in pH 7.0 20 mmol.L-1 phosphate buffer, and reacted at 60 degrees C for 48h. Then, ara-DA was effectively transformed into ara-G with adenylate deaminase produced by Aspergillus oryzae DAW-01. The total process had no complex separation and purification.