文献类型：期刊文献

英文题名：Engineering Pseudomonas putida KT2440 for simultaneous degradation of carbofuran and chlorpyrifos

作者：Gong, Ting[1,2];Liu, Ruihua[1,2];Che, You[1,2];Xu, Xiaoqing[1,2];Zhao, Fengjie[1,2];Yu, Huilei[3];Song, Cunjiang[1,2];Liu, Yanping[4];Yang, Chao[1,2]

机构：[1]Nankai Univ, Minist Educ, Key Lab Mol Microbiol & Technol, Tianjin 300071, Peoples R China;[2]Nankai Univ, State Key Lab Med Chem Biol, Tianjin 300071, Peoples R China;[3]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[4]Tianjin Med Univ, Gen Hosp, Dept Gynaecol & Obstet, Tianjin 300052, Peoples R China

年份：2016

卷号：9

期号：6

起止页码：792

外文期刊名：MICROBIAL BIOTECHNOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000387000900009)】；

基金：This study was supported by National Key Technology Support Program of China (no. 2015BAD16B04), National Natural Science Foundation of China (nos. 31300032 and 31570035), Open Fund of State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences (no. SKLMR-20130604), Open Fund of State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology and Ph.D. Candidate Research Innovation Fund of Nankai University.

语种：英文

摘要：Currently, chlorpyrifos (CP) and carbofuran are often applied together to control major agricultural pests in many developing countries, in most cases, they are simultaneously detected in agricultural soils. Some cost-effective techniques are required for the remediation of combined pollution caused by multiple pesticides. In this work, we aim at constructing a detectable recombinant microorganism with the capacity to simultaneously degrade CP and carbofuran. To achieve this purpose, CP/carbofuran hydrolase genes and gfp were integrated into the chromosome of a biosafety strain Pseudomonas putida KT2440 using a chromosomal scarless modification strategy with upp as a counter-selectable marker. The toxicity of the hydrolysis products was significantly lower compared with the parent compounds. The recombinant strain could utilize CP or carbofuran as the sole source of carbon for growth. The inoculation of the recombinant strain to soils treated with carbofuran and CP resulted in a higher degradation rate than in noninoculated soils. Introduced green fluorescent protein can be employed as a biomarker to track the recombinant strain during bioremediation. Therefore, the recombinant strain has potential to be applied for insitu bioremediation of soil co-contaminated with carbofuran and CP.