文献类型：期刊文献

英文题名：Rationally optimized generation of integrated Escherichia coli with stable and high yield lycopene biosynthesis from heterologous mevalonate (MVA) and lycopene expression pathways

作者：Hussain, Muhammad Hammad[1];Hong, Qi[1];Zaman, Waqas Qamar[2];Mohsin, Ali[1];Wei, Yanlong[1];Zhang, Ning[1];Fang, Hongqing[1,3];Wang, Zejian[1];Hang, Haifeng[1];Zhuang, Yingping[1];Guo, Meijin[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]Natl Univ Sci & Technol NUST, Sch Civil & Environm Engn, Inst Environm Sci & Engn, Sect H-12, Islamabad 44000, Pakistan;[3]Beijing Inst Biotechnol, 20 Dongda St, Beijing 100071, Peoples R China

年份：2021

卷号：6

期号：2

起止页码：85

外文期刊名：SYNTHETIC AND SYSTEMS BIOTECHNOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000661498100005)】；

基金：This research was supported by grants from the National Key Research and Development Program of China (2018YFA0900300 and 2019YFA090141) and the National Science and Technology Major Project (2017ZX07402003).

语种：英文

外文关键词：Escherichia coli; Lycopene biosynthesis; Rational model of expression pathways; Integrated MVA pathway; Fermentation

摘要：The stability and high productivity of heterogeneous terpenoid production in Escherichia coli expression system is one of the most key issues for its large scale industrialization. In the current study on taking lycopene biosynthesis as an example, an integrated Escherichia coli system has been generated successfully, which resulted into stable and high lycopene production. In this process, two modules of mevalonate (MVA) pathway and one module of lycopene expression pathway were completely integrated in the chromosome. Firstly, the copy number and integrated position of three modules of heterologous pathways were rationally optimized. Later, a strain DH416 equipped with heterogeneous expression pathways through chromosomal integration was efficiently derived from parental strain DH411. The evolving DH416 strain efficiently produced the lycopene level of 1.22 g/L (49.9 mg/g DCW) in a 5 L fermenter with mean productivity of 61.0 mg/L/h. Additionally, the integrated strain showed more genetic stability than the plasmid systems after successive 21st passage.