文献类型：期刊文献

英文题名：Quantitative analysis of hemoglobin content in polymeric nanoparticles as blood substitutes using Fourier transform infrared spectroscopy

作者：Shan, Xiaoqian[1,2];Chen, Ligen;Yuan, Yuan[1,2];Liu, Changsheng[1,2];Zhang, Xiaolan[1,2];Sheng, Yan[1,2];Xu, Feng[1,2]

机构：[1]E China Univ Sci & Technol, Key Lab Ultrafine Mat, Minist Educ, Shanghai 200237, Peoples R China;[2]E China Univ Sci & Technol, Engn Res Ctr Biomed Mat, Minist Educ, Shanghai 200237, Peoples R China

年份：2010

卷号：21

期号：1

起止页码：241

外文期刊名：JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE

收录：;EI(收录号：20100412671329);WOS:【SCI-EXPANDED(收录号:WOS:000273592200027)】；

基金：The authors acknowledge the financial support from the National High Technology Research and Development Program of China (863 program) (No. 2004AA-302050) and from Shanghai Nanotechnology Special Foundation (No. 0452nm022).

语种：英文

外文关键词：Emulsification - Calibration - Ethylene glycol - Polyols - Drug delivery - Fourier transform infrared spectroscopy - Nanoparticles - Polyethylene glycols

摘要：Based on the penetrability of IR within the polymeric nanoparticles, a novel Fourier transform infrared spectroscopy (FTIR) method, with polyacrylonitrile (PAN) as the internal reference standard, was developed to quantify the hemoglobin (Hb) content in Hb-based polymeric nanoparticles (HbPN). The HbPN was fabricated by double emulsion method from poly(ethylene glycol)-poly(lactic acid)-poly(ethylene glycol) triblock copolymers. Depending on the characteristic un-overlapped IR absorbances at 1540 cm(-1) of Hb (amide II) and at 2241 cm(-1) of PAN (-Ca parts per thousand N), calibration equations, presenting the peak height ratio of Hb and PAN as a function of the weight ratio of Hb and PAN, were established. This new quantification method is validated and used to the determination Hb content in HbPN. Due to the good results of this calibration strategy, the proposed simple FTIR approach with minimal sample-needed and solvent-free makes it useful for routine analysis of protein content and could be also applied to any other drug/protein encapsulated particles.