文献类型：期刊文献

中文题名：不对称水溶性菁染料的简便制备与纯化方法

英文题名：Synthesis and Purification of Unsymmetrical Water-soluble Cyanine Dyes

作者：楼开炎[1];钱旭红[1];宋恭华[1]

机构：[1]华东理工大学药物化工研究所,上海200237

年份：2002

卷号：28

期号：2

起止页码：212

中文期刊名：华东理工大学学报（自然科学版）

外文期刊名：Journal of East China University of Science and Technology

收录：CSTPCD;;Scopus;北大核心:【北大核心2000】；CSCD:【CSCD2011_2012】；

语种：中文

中文关键词：水溶性菁染料;合成;纯化;制备薄层色谱

外文关键词：water soluble cyanine; synthesis; purification; PTLC

摘要：以对肼基苯磺酸为起始原料 ,经 6步反应合成了 2个可用于 DNA荧光标记的不对称水溶性菁染料。采用三元展开剂体系 ,用制备薄层色谱方法分离提纯各中间体和产物 。
Two water soluble unsymmetrical cyanine dyes, which can be used as fluorescence tag in DNA labeling, was synthesized from p hydrazino benzenesulfonic acid by 6 steps. A ternary solvent were developed and used as eluant in purification of intermediates and final dyes by PTLC method. The yields were as good as that of the HPLC method that had been reported.