文献类型：期刊文献

英文题名：Engineered Bacillus subtilis Biofilm@Biochar living materials for in-situ sensing and bioremediation of heavy metal ions pollution

作者：Zhu, Xiaojuan[1,2];Xiang, Qinyuan[1,2];Chen, Lin[1,2];Chen, Jianshu[1,2];Wang, Lei[1,2];Jiang, Ning[1,2];Hao, Xiangrui[3];Zhang, Hongyan[3];Wang, Xinhua[4];Li, Yaqian[4];Omer, Rabia[1,2];Zhang, Lingfan[5];Wang, Yonghong[1];Zhuang, Yingping[1,2];Huang, Jiaofang[1,6,7]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Shanghai Collaborat Innovat Ctr Biomfg SCICB, Shanghai 200237, Peoples R China;[3]Shanghai Nong Biol Prod Co Ltd NLBP, Shanghai 201419, Peoples R China;[4]Shanghai Jiao Tong Univ, Sch Agr & Biol, Shanghai 200240, Peoples R China;[5]East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai 200237, Peoples R China;[6]Jiangxi Normal Univ, Coll Life Sci, Nanchang 330022, Peoples R China;[7]East China Univ Sci & Technol, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2024

卷号：465

外文期刊名：JOURNAL OF HAZARDOUS MATERIALS

收录：;EI(收录号：20235215275546);WOS:【SCI-EXPANDED(收录号:WOS:001142629500001)】；

基金：This study was supported by the National Key Research and Devel- opment Program of China (grant no. 2020YFA0908900) , the Natural Science Foundation of Shanghai (grant no. 22ZR1416000) , and the Fundamental Research Funds for the Central Universities. We thank the Research Center of Analysis and Test (ECUST) for the assistance pro- vided, in particular engineers L.F. Zhang and Y. Q. Xi for supplying the ICP-OES data. We thank Professor Fener Chen, Chao Zhong, Rubin Liang and Ali Mohsin for their constructive comments of the paper.

语种：英文

外文关键词：Heavy metal ions pollution; Biosensor; In-situ sensing; Green remediation; B. subtilis biofilm; Engineered living materials

摘要：The simultaneous sensing and remediation of multiple heavy metal ions in wastewater or soil with microor-ganisms is currently a significant challenge. In this study, the microorganism Bacillus subtilis was used as a chassis organism to construct two genetic circuits for sensing and adsorbing heavy-metal ions. The engineered biosensor can sense three heavy metal ions (0.1-75 mu M of Pb2+ and Cu2+, 0.01-3.5 mu M of Hg2+) in situ real-time with high sensitivity. The engineered B. subtilis TasA-metallothionein (TasA-MT) biofilm can specifically adsorb metal ions from the environment, exhibiting remarkable removal efficiencies of 99.5% for Pb2+, 99.9% for Hg2+and 99.5% for Cu2+ in water. Furthermore, this engineered strain (as a biosensor and absorber of Pb2+, Cu2+, and Hg2+) was incubated with biochar to form a hybrid biofilm@biochar (BBC) material that could be applied in the bioremediation of heavy metal ions. The results showed that BBC material not only significantly reduced exchangeable Pb2+ in the soil but also reduced Pb2+ accumulation in maize plants. In addition, it enhanced maize growth and biomass. In conclusion, this study examined the potential applications of biosensors and hybrid living materials constructed using sensing and adsorption circuits in B. subtilis, providing rapid and cost-effective tools for sensing and remediating multiple heavy metal ions (Pb2+, Hg2+, and Cu2+).