文献类型：期刊文献

中文题名：鸡卵黄抗体(IgY)的诱导

英文题名：Induction of Egg Yolk Antibody(IgY)

作者：范龙彬[1];杨曜中[1];俞建瑛[1];宋聿文[1];欧伶[1]

机构：[1]华东理工大学应用生物系,上海200237

年份：2000

卷号：13

期号：2

起止页码：79

中文期刊名：中国生物制品学杂志

外文期刊名：Chinese Journal of Biologicals

收录：CSTPCD;;Scopus;CSCD:【CSCD2011_2012】；

语种：中文

中文关键词：鸡;卵黄抗体;免疫诱导;抗体滴度;卵黄液

外文关键词：Eggyolk IgY Morphine Bovine serum albuminP

摘要：目的　获得鸡卵黄抗体（Ｉｇｙ），并研究该抗体在鸡的黄液中表达的进程。方法　用吗啡—牛血清蛋白 结合物为模型抗原对３０周龄产蛋鸡进行免疫诱导，用ＥＬＩＳＡ间接法测定不同时间的抗体滴度。结果全程免疫过 程中白蛋白抗体最高滴度为１：１２８００，吗啡抗体最高滴度为 １：１６００，经 ５次免疫后（４２周龄）抗体滴度不再升高。结论　为使目的抗体在鸡卵黄液中持续而高质量诱导奠定基础。
Objective To obtain IgY and study on the expression of it in egg yolk,Methods The laying hens aged 3Ow were immunized with morphine-bovine serum albumin conjugate,and the IgY induced was detected by indirect ELISA.Results The highest biers of antibodies to albumin and morphine were 1: 12800 and 1:1600 respectively. However, no increase of the biers were observed in the hens(aged 42w)after being immunized with 5 doses of the conjugate.Conclusion The study laid a foundation of the induction and persistence of goal antibody in egg yolk.