文献类型：期刊文献

英文题名：Scale-down of Cephalosporium acremonium Cultivations for High Throughput Screening of High-yield Cephalosporin C Strains.

作者：Ngoc Thang Vu[1,2];Tan, Jun[1];Ahmed, Mohamed Bedair[4];Qambrani, Naveed Ahmed[5];Hao, Yu You[3];Zhang, Si Liang[1];Chu, Ju[1]

机构：[1]East China Univ Sci & Technol, Natl Engn Res Ctr Biotechnol Shanghai, Shanghai, Peoples R China;[2]Vietnam Natl Univ Agr, Fac Agron, Hanoi, Vietnam;[3]Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Plant Physiol & Ecol, Shanghai 200232, Peoples R China;[4]Natl Res Ctr, Dept Food Toxicol & Contaminants, Cairo, Egypt;[5]Kangwon Natl Univ, Coll Agr & Life Sci, Dept Biol Environm, Chunchon, South Korea

年份：2016

卷号：7

期号：4

起止页码：22

外文期刊名：RESEARCH JOURNAL OF PHARMACEUTICAL BIOLOGICAL AND CHEMICAL SCIENCES

收录：WOS:【ESCI(收录号:WOS:000410760200004)】；

基金：This research was supported by Open Funding Project of the State Key Laboratory of Bioreactor Engineering. The authors of this paper would like to thank all professors in National Engineering Research Center for Biotechnology (Shanghai) of East China University of Science and Technology for their valuable suggestions and helpful comments.

语种：英文

外文关键词：Cephalosporium acremonium; cephalosporin C; high throughput screening; scale-down; strain improvement

摘要：A high throughput screening (HIS) strategy was investigated for screening high-yielding cephalosporin C (CPC) using mutated Cephalosporium acremonium. The strategy was based on microtiter plates (MTPs) to culture the microorganisms and measure its products. The feasibility of MTPs was to replace the cultivation in shake flasks, and CPC production will be determined using turbidimetric assay by ELISA reader to replace HPLC assay. The HIS systems were verified by screening high-producing CPC mutants. By using this HTS strategy, two mutants, i.e. 5-Al and 8-A2 were selectedf m the secondary mutagenesis. The volumetric productivity of Cephalosporin C from Cephalosporium acremonium was 7914.43 U/nnk 8639.24 U/nnl in shake flasks. CPC production was improved about 246% and 269% compared with that of original strain.