文献类型：期刊文献

中文题名：银杏叶提取物中槲皮素和芦丁的超临界流体色谱法测定

英文题名：Determination of Quercetin and Rutin in Ginkgo biloba Extract by Supercritical Fluid Chromatography

作者：王学军[1];许振良[1];赵锁奇[2]

机构：[1]华东理工大学化学工程研究所,上海200237;[2]中国石油大学重质油国家重点实验室,北京102249

年份：2005

卷号：36

期号：7

起止页码：415

中文期刊名：中国医药工业杂志

外文期刊名：Chinese Journal of Pharmaceuticals

收录：CSTPCD;;北大核心:【北大核心2004】；CSCD:【CSCD2011_2012】；

语种：中文

中文关键词：银杏叶提取物;槲皮素;芦丁;黄酮;超临界流体色谱;测定

外文关键词：Ginkgo biloba extract; quercetin; rutin; flavonoids; supercritical fluid chromatography; determination

摘要：建立了超临界流体色谱法同时测定银杏叶提取物中槲皮素和芦丁的含量。采用C18色谱柱,流动相为超临界CO2-0.05%三氟乙酸的乙醇溶液(10∶1),检测波长360nm。槲皮素和芦丁的平均回收率分别为99.2%和101.3%,RSD分别为2.3%和2.8%。并考察了流动相组成、温度和压力对色谱分离的影响。
A supercritical fluid chromatography for determination of quercetin and rutin in Ginkgo biloba extractwas established. A C18 column was used with the mobile phase of supercritical CO2-0.05% trifluoroacetic acid inethanol (10∶1) at the detection wavelength of 360nm. The average recoveries of quercetin and rutin were 99.2% (RSD2.3%) and 101.3% (RSD 2.8%), respectively. The influences of composition of mobile phase, temperature and pressurewere also discussed.