文献类型：期刊文献

英文题名：Surface properties of amino-functionalized poly(ε-caprolactone) membranes and the improvement of human mesenchymal stem cell behavior

作者：Zhang, Yi[1];Zhang, Yan[1];Chen, Min[2];Yan, Jinliang[1];Ye, Zhaoyang[2];Zhou, Yan[2];Tan, Wensong[2];Lang, Meidong[1]

机构：[1]E China Univ Sci & Technol, Sch Mat Sci & Engn, Minist Educ, Key Lab Ultrafine Mat, Shanghai 200237, Peoples R China;[2]E China Univ Sci & Technol, Sch Bioengn, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2012

卷号：368

期号：1

起止页码：64

外文期刊名：JOURNAL OF COLLOID AND INTERFACE SCIENCE

收录：;EI(收录号：20120314699054);WOS:【SCI-EXPANDED(收录号:WOS:000299490500009)】；

基金：Financial support from the Fundamental Research Funds for the Central Universities (WD0913008 and WD1014036), the National Natural Science Foundation of China (20804015), Specialized Research Fund for the Doctoral Program of Higher Education (200802511021), Shanghai Key Laboratory Project (08DZ2230500), and Program for Changjiang Scholars and Innovative Research Team in University (IRT0825) are gratefully acknowledged.

语种：英文

外文关键词：Functionalized poly(epsilon-caprolactone); Amino groups; Surface properties; hMSC

摘要：In this study, a series of membranes with different amino group densities were prepared to investigate the surface properties of the novel poly(gamma-amino-epsilon-caprolactone-co-epsilon-caprolactone) (NPCL) copolymer synthesized by our laboratory. Meanwhile, the human mesenchymal stem cells' (hMSCs) behavior on those membranes was examined. The molecular characteristics of the NPCL copolymers were characterized by nuclear magnetic resonance (NMR), size exclusion chromatography (SEC), and differential scanning calorimetry (DSC). Surface properties of membranes were characterized by water contact angle analysis, X-ray photoelectron spectroscopy analysis (XPS), and atomic force microscopy (AFM). It was found that the incorporation of amino groups to the poly(epsilon-caprolactone) (PCL) backbone resulted in an augmented wettability, a decreased crystallinity, and also an increased surface roughness on the NPCL membranes. In vitro cell experiments showed a significant enhancement in hMSCs' adhesion, proliferation, and osteogenic differentiation on NPCL membranes compared with virgin PCL membrane, and demonstrated that surface properties of membrane played an important role in tailoring cell behavior. (C) 2011 Elsevier Inc. All rights reserved.