文献类型：期刊文献

中文题名：肝素黄杆菌发酵产肝素酶的工艺优化

英文题名：Optimization of Heparinase Production by Flavobacterium heparinum

作者：马小来[1];伍炀[1];顾含真[1];袁勤生[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室,上海200237

年份：2005

卷号：31

期号：4

起止页码：446

中文期刊名：华东理工大学学报（自然科学版）

外文期刊名：Journal of East China University of Science and Technology

收录：CSTPCD;;EI(收录号：2005389375477);Scopus;北大核心:【北大核心2004】；CSCD:【CSCD2011_2012】；

基金：上海市重点学科资助项目

语种：中文

中文关键词：肝素酶;肝素黄杆菌;发酵

外文关键词：Flavobacterium heparinum ; heparinase ; fermentation

摘要：以肝素黄杆菌为研究对象,提出了一种将发酵分为生长和产酶两个阶段的生产肝素酶的新工艺。对培养基组成进行了优化,优化后组成(g/L)葡萄糖8,氯化铵4,磷酸二氢盐6,组氨酸0.75,甲硫氨酸0.75,氯化镁0.5;微量盐10-4mol/L。菌体生长和产酶两个阶段的最优温度为27°C和23°C,pH为6.4和7.0。在诱导时加入10-4mol/LBa2+可以较好地消除SO2-4的阻遏作用。采用优化工艺,摇瓶产酶比原来提高了66%。
A novel fermentation processes to produce heparinase by Flavobacterium heparinum were established. The fermentation is divided into two stages： bacteria growth and enzyme production. The optimized culture medium composition were as follows（g/L）, glucose 8, NH4Cl 4, NaH2PO4 3, KH2PO4 3,histidine 0.75, methinine 0.75, MgCl2 0.5 and trace elements 10^-4 mol/L. The optimum temperatures and pH for bacteria growth and enzyme production were 27 ℃ and 23 ℃, and 6.4 and 7.0 respectively. Addition of Ba^2＋ at 10^-4 mol/L could get rid of sulfur repression which led to the increase of the total enzyme productivity of heparinase by 66 %.