文献类型：期刊文献

中文题名：胡黄连甙II和神经生长因子对H_2O_2损伤的PC12细胞的协同保护作用(英文)

英文题名：Synergistic protective effect of picroside II and NGF on PC12 cells against oxidative stress induced by H_2O_2

作者：张小东[1];刘建文[1];李婷[1];曹艳[1];郭明川[1]

机构：[1]华东理工大学药学院,上海200237

年份：2007

卷号：12

期号：1

起止页码：32

中文期刊名：中国临床药理学与治疗学

外文期刊名：Chinese Journal of Clinical Pharmacology and Therapeutics

收录：CSTPCD;;CSCD:【CSCD2011_2012】；

基金：Shanghai SK-foundation project(2003008t)

语种：中文

中文关键词：双氧水;神经生长因子;胡黄连甙Ⅱ;活性氧

外文关键词：hydrogen peroxide; nerve growth factor; picreside Ⅱ; reactive oxygen specie

摘要：目的:考察胡黄连甙II和NGF对双氧水损伤细胞的协同保护作用。方法:以CDCFH方法评价活性氧水平,MTT方法、细胞形态观察以及LDH漏出实验评价药物对细胞损伤的修复作用。结果:25μg/mL胡黄连甙II和2 ng/mL NGF显著改善H2O2诱导的PC12细胞损伤,同时有效降低细胞内ROS。细胞形态改善、细胞活力提高、ROS清除作用均表明胡黄连甙II和NGF协同保护氧化应激对神经细胞的损伤。结论:胡黄连甙II和NGF的协同作用能有效治疗神经性疾病。
AIM： To study the synergistic protective effect of picroside Ⅱ and NGF for the oxidative stress on PC12 cells induced by hydrogen peroxide （H202）. METHODS： The fluorescent probe 6-carboxy-2＇, 7＇-dichlorodihydr of luorescein （CDCFH） was used to assess the intracellular reactive oxygen species （ROS） level, and MTT assay, morphological observation as well as lactate dehydrogenase （LDH） leakage were conducted to measure cellular injury. RESULTS： The H2O2-induced cytotoxicity was significantly attenuated in the presence of picroside Ⅱ （25 μg/mL） mM NGF （2 ng/mL）. Cultures with this combined treatment possessed decreased level of ROS while increased cell survival, as compared to that of picroside Ⅱ or NGF alone-treated cells. Accordingly, it was concluded that their synergistic protective activities against oxidative stress in vitro were demonstrated in various aspects including reversing morphological changes, enhancing the ability of cell proliferation and ROS scavenging. CONCLUSION： Such action supports the therapeutic potential of picroside Ⅱ and NGF in treating nervous disorders based on their synergistic effect.