文献类型：期刊文献

英文题名：Correlation of antibody production rate with glucose and lactate metabolism in Chinese hamster ovary cells

作者：Chen, Fei[1];Ye, Zhaoyang[1];Zhao, Liang[1];Liu, Xuping[1];Fan, Li[1];Tan, Wen-Song[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2012

卷号：34

期号：3

起止页码：425

外文期刊名：BIOTECHNOLOGY LETTERS

收录：;EI(收录号：20120614749033);WOS:【SCI-EXPANDED(收录号:WOS:000301296300003)】；

基金：This work was supported by the East China University of Science and Technology (ECUST) Special Research Fund for Outstanding Young Scholars (No. YF0157127).

语种：英文

外文关键词：Antibody production; Cell metabolism; Chinese hamster ovary cells; Mitochondrial dehydrogenase activity

摘要：A linear relationship was found between the antibody production rate (q(mAb)) and the glucose and lactate consumption rate (q(GL)) in Chinese hamster ovary cells. Under a series of q(mAb)-perturbing conditions, q(GL) was determined and a linear relationship between q(mAb) and q(GL) was further established (R-2 = 0.914). Mitochondrial dehydrogenase activity was monitored in all the qmAb-perturbing conditions and showed a linear correlation with q(GL) (R-2 = 0.874) as well as with q(mAb). Taken collectively, our results establish that the metabolic parameter, q(GL), is linearly correlated with q(mAb); this finding strengthens our current understanding of process optimization for antibody production.