文献类型：期刊文献

英文题名：A highly sensitive long-wavelength fluorescence probe for nitroreductase and hypoxia: selective detection and quantification

作者：Guo, Ting[1];Cui, Lei[1];Shen, Jiaoning[1];Zhu, Weiping[1];Xu, Yufang[1];Qian, Xuhong[1]

机构：[1]E China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab Chem Biol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2013

卷号：49

期号：92

起止页码：10820

外文期刊名：CHEMICAL COMMUNICATIONS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000326219500016)】；

基金：The authors are grateful for the financial support from the State Key Program of National Natural Science of China (21236002), the National Basic Research Program of China (2010CB126100), the National High Technology Research and Development Program of China (2011AA10A207), and the Fundamental Research Funds for the Central Universities.

语种：英文

摘要：A novel long-wavelength fluorescence probe NBP has been developed for the detection of nitroreductase (NTR) and hypoxia. NBP could be activated by NTR at 0.1 mu M to release the fluorophore NBF and significant changes in fluorescence emission at 658 nm were observed. This feature makes it advantageous for imaging hypoxic cells with minimal endogenous interference.