文献类型：期刊文献

英文题名：Discarded free PEG-based assay for obtaining the modification extent of pegylated proteins

作者：Gong, Xing Wen[1]; Wei, Dong Zhi[1]; He, Ming Lei[1]; Xiong, Yu Chun[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Inst New World Biotechnol, Shanghai 200237, Peoples R China

年份：2007

卷号：71

期号：1

起止页码：381

外文期刊名：TALANTA

收录：;EI(收录号：20070210354974);WOS:【SCI-EXPANDED(收录号:WOS:000244059600057)】；

语种：英文

外文关键词：polyethylene glycol; pegylated protein; free PEG; modification extent

摘要：Free polyethylene glycol (PEG) is a byproduct produced during the process of pegylation and should be removed for the purification of pegylated proteins. In this paper, it was used to develop a new method for obtaining the modification extent of pegylated proteins. This method included two steps of operation. Firstly, the free PEG was separated from crude reaction mixture of pegylated proteins by CM-Sepharose FF. Then PEG was determined based on the formation of a complex with barium chloride and iodine solution. The effective detective range of PEG was 0-7.5 mu g/ml. The modification extent was calculated according to a formula. This method is simple, sensitive, and applicable to all of the PEG derivatives. The most distinctive aspect is that it does not consume proteins. (c) 2006 Elsevier B.V. All rights reserved.