文献类型：期刊文献

英文题名：Current Advances in 3D Dynamic Cell Culture Systems

作者：Huang, Xin[1];Huang, Zhengxiang[1,2];Gao, Weidong[1];Gao, Wendong[1];He, Ruiying[3];Li, Yulin[4];Crawford, Ross[1,5];Zhou, Yinghong[5,6];Xiao, Lan[1,5];Xiao, Yin[1,5,7]

机构：[1]Queensland Univ Technol, Ctr Biomed Technol, Sch Mech Med & Proc Engn, Brisbane, Qld 4059, Australia;[2]Univ Queensland, Sch Biomed Sci, St Lucia, Qld 4072, Australia;[3]Hubei Univ, Coll Chem & Chem Engn, Wuhan 430062, Peoples R China;[4]East China Univ Sci & Technol, Engn Res Ctr Biomed Mat, Sch Mat Sci & Engn, Key Lab Ultrafine Mat,Minist Educ,State Key Lab Bi, Shanghai 200237, Peoples R China;[5]Queensland Univ Technol, Australia China Ctr Tissue Engn & Regenerat Med, Brisbane, Qld 4059, Australia;[6]Univ Queensland, Sch Dent, Herston, Qld 4072, Australia;[7]Griffith Univ, Sch Med & Dent, Gold Coast, Qld 4222, Australia

年份：2022

卷号：8

期号：12

外文期刊名：GELS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000902427100001)】；

基金：This research is supported by the Joint Research Centre Fund from the Department of Environment and Science (2019-2023), Queensland, Australia.

语种：英文

外文关键词：3D cell culture; mechanical stimulation on cell behavior; bioreactor; microcarrier; organ-on-a-chip

摘要：The traditional two-dimensional (2D) cell culture methods have a long history of mimicking in vivo cell growth. However, these methods cannot fully represent physiological conditions, which lack two major indexes of the in vivo environment; one is a three-dimensional 3D cell environment, and the other is mechanical stimulation; therefore, they are incapable of replicating the essential cellular communications between cell to cell, cell to the extracellular matrix, and cellular responses to dynamic mechanical stimulation in a physiological condition of body movement and blood flow. To solve these problems and challenges, 3D cell carriers have been gradually developed to provide a 3D matrix-like structure for cell attachment, proliferation, differentiation, and communication in static and dynamic culture conditions. 3D cell carriers in dynamic culture systems could primarily provide different mechanical stimulations which further mimic the real in vivo microenvironment. In this review, the current advances in 3D dynamic cell culture approaches have been introduced, with their advantages and disadvantages being discussed in comparison to traditional 2D cell culture in static conditions.