文献类型：期刊文献

英文题名：Multiple gene integration to promote erythritol production on glycerol in Yarrowia lipolytica

作者：Zhang, Ling[1];Nie, Ming-Yue[1];Liu, Feng[1];Chen, Jun[1];Wei, Liu-Jing[1];Hua, Qiang[1,2]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]Shanghai Collaborat Innovat Ctr Biomfg Technol, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2021

卷号：43

期号：7

起止页码：1277

外文期刊名：BIOTECHNOLOGY LETTERS

收录：;EI(收录号：20211510184833);WOS:【SCI-EXPANDED(收录号:WOS:000636158300004)】；

基金：This work was supported by National Key R&D Program of China (2017YFE0115600, 2019YFA0904302) and the 111 Project (B18022).

语种：英文

外文关键词：Erythritol; Glycerol utilization; Multiple gene integration; Ribose 5-phosphate isomerase; Yarrowia lipolytica

摘要：Objective Erythritol (1,2,3,4-butanetetrol) is a 4-carbon sugar alcohol that occurs in nature as a metabolite or storage compound. In this study, a multiple gene integration strategy was employed to enhance erythritol production in Y. lipolytica. Results The effects on the production of erythritol in Y. lipolytica of seven key genes involved in the erythritol synthesis pathway were evaluated individually, among which transketolase (TKL1) and transaldolase (TAL1) showed important roles in enhancing erythritol production. The combined overexpression of four genes (GUT1, TPI1, TKL1, TAL1) and disruption of the EYD1 gene (encoding erythritol dehydrogenase), resulted in produce approximately 40 g/L erythritol production from glycerol. Further enhanced erythritol synthesis was obtained by overexpressing the RKI1 gene (encoding ribose 5-phosphate isomerase) and the AMPD gene (encoding AMP deaminase), indicating for the first time that these two genes are also related to the enhancement of erythritol production in Y. lipolytica. Conclusions A combined gene overexpression strategy was developed to efficiently improve the production of erythritol in Y. lipolytica, suggesting a great capacity and promising potential of this non-conventional yeast in converting glycerol into erythritol.