文献类型：期刊文献

中文题名：一种新型纳米通道单分子检测探头的设计

英文题名：Design of a Novel Sensing Probe for Nanopore Single-Molecule Detection

作者：颜秉勇[1];朱明乔[1];王学武[1]

机构：[1]华东理工大学信息科学与工程学院,上海200237

年份：2018

卷号：44

期号：6

起止页码：869

中文期刊名：华东理工大学学报（自然科学版）

外文期刊名：Journal of East China University of Science and Technology

收录：CSTPCD;;Scopus;北大核心:【北大核心2017】；CSCD:【CSCD_E2017_2018】；

基金：国家重大科研仪器研制专项(21327807);国家自然科学基金青年基金(51407078);国家自然科学基金面上项目(61773165)

语种：中文

中文关键词：纳米通道单分子检测;前置补偿;动态响应;零点;极点

外文关键词：nanopore single-molecule detection;precompensation;dynamic response;zero point;pole point

摘要：针对纳米通道单分子检测的探头测量系统,在详细分析探头结构以及测量带宽等属性的基础上,采用前置带宽补偿的原理,提出了一种改进的检测探头的设计方法。该方法不仅能够扩大原有测量系统的测量带宽,而且能够灵活配置系统的零点和极点,加快系统的动态响应过程。通过仿真实验验证了本文方法的可行性和有效性,并且通过实际测量探头电路给出了测量实验结果。
By analyzing the properties of probe measurement system for single molecule detection of nanopore in detail,mainly including the structure of the probe and the measurement bandwidth,this paper designs an improved high-precision acquisition probe system by utilizing the pre-compensation method.The objective of the acquisition probe system is the efficient acquisition of the original signal in nanopore single-molecule detection system,in which the input signal is pA-level current.In order to measure small current signals,it is necessary to use a high-gain operational amplifier as widely used in traditional nanopore probe system.However,there are two main shortcomings in traditional nanopore probe system,i.e.,the insufficiency of the system measurement bandwidth(only a few kHz)and too much noise in the current signal.These are mainly due to the value of the feedback resistance in the operational amplifier circuit to be too huge(usually reaching a few GΩ).The parasitic capacitance of the large resistor will affect the bandwidth of system and the thermal noise of the large resistor will increase the circuit noise of the system.Especially,the resistance value has positive correlation with the noise of the circuit and negative correlation with the bandwidth of the system.Hence,it is quite important to select an appropriate resistance value when we consider these factors.The value of the feedback resistance in the designed probe system is200MΩ.The designed acquisition probe system can not only expand the measurement bandwidth of the original measurement system,but also flexibly configure zero point and pole point of the system to speed up the dynamic response process of the system.Finally,the feasibility and effectiveness of the proposed method are verified by simulation experiments through the actual probe circuit.