文献类型：期刊文献

英文题名：Molecular phylogenetic diversity of the microbial community associated with a high-temperature petroleum reservoir at an offshore oilfield

作者：Li, Hui; Yang, Shi-Zhong; Mu, Bo-Zhong; Rong, Zhao-Feng; Zhang, Jie

机构：[1]E China Univ Sci & Technol, Inst Appl Chem, Shanghai 200237, Peoples R China;[2]E China Univ Sci & Technol, Inst Bioengn, Shanghai, Peoples R China

年份：2007

卷号：60

期号：1

起止页码：74

外文期刊名：FEMS MICROBIOLOGY ECOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000244883800008)】；

语种：英文

外文关键词：petroleum reservoir; 16S rRNA gene diversity; microbial community; clone library

摘要：The microbial community and its diversity in production water from a high-temperature, water-flooded petroleum reservoir of an offshore oilfield in China were characterized by 16S rRNA gene sequence analysis. The bacterial and archaeal 16S rRNA gene clone libraries were constructed from the community DNA and, using sequence analysis, 388 bacterial and 220 archaeal randomly selected clones were clustered with 60 and 28 phylotypes, respectively. The results showed that the 16S rRNA genes of bacterial clones belonged to the divisions Firmicutes, Thermotogae, Nitrospirae and Proteobacteria, whereas the archaeal library was dominated by methanogen-like rRNA genes (Methanothermobacter, Methanobacter, Methanobrevibacter and Methanococcus), with a lower percentage of clones belonging to Thermoprotei. Thermophilic microorganisms were found in the production water, as well as mesophilic microorganisms such as Pseudomonas and Acinetobacter-like clones. The thermophilic microorganisms may be common inhabitants of geothermally heated specialized subsurface environments, which have been isolated previously from a number of high-temperature petroleum reservoirs worldwide. The mesophilic microorganisms were probably introduced into the reservoir as it was being exploited. The results of this work provide further insight into the composition of microbial communities of high-temperature petroleum reservoirs at offshore oilfields.