文献类型：期刊文献

英文题名：A novel method for extraction of paramylon from Euglena gracilis for industrial production

作者：Zhang, Kaikai[1];Wan, Minxi[1,3];Bai, Wenmin[2];He, Maolei[2];Wang, Weiliang[1];Fan, Fei[1];Guo, Jiacai[1];Yu, Tao[1];Li, Yuanguang[1,3]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Baoshan Zeyuan Algae Hlth Technol Co Ltd, Baoshan 678208, Peoples R China;[3]Mai Box 301,Meilong Rd 130, Shanghai 200237, Peoples R China

年份：2023

卷号：71

外文期刊名：ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000953753300001)】；

基金：Acknowledgements This research was supported by the National Natural Science Foun-dation of China (21706071) .

语种：英文

外文关键词：Paramylon; Disc stack centrifuge; E; gracilis concentrate; SDS; Response surface methodology; Pilot-scale extraction

摘要：For establishing an extraction process of paramylon for industrial production, the process of disrupting and concentrating Euglena gracilis cells by disc stack centrifuge (DSC), and then paramylon was extracted successfully on the pilot scale by the method of sodium dodecyl sulfate (SDS) in this study. The results revealed that the cell disruption rate of 93.44 % could be obtained by disc stack centrifuge. The optimal extraction conditions of paramylon in E. gracilis concentrate were SDS addition of 5.4 g L-1, extraction temperature of 76 degrees C, single extraction time of 42 min, and the number of extraction times of 2. There was no photosynthetic pigment residue in the extract before washing. Furthermore, washing 3 times could effectively reduce the SDS residue in para-mylon. The content of paramylon extracted by the pilot process is >97 %. The extraction yield can reach >92 %. There are no cytochrome and SDS residues in paramylon. The extraction process of paramylon established in this study is convenient, free of organic solvent, and easy to operate and scale up.