文献类型：期刊文献

英文题名：Feedback-induced phase separation of hollow condensates to create biomimetic membraneless compartments

作者：Fu, Jianmian[1];Wang, Bin[1];Zhu, Weiping[1,3];Xu, Yufang[1];Qian, Xuhong[1,2];Yang, Yangyang[1]

机构：[1]East China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab Chem Biol, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[3]East China Univ Sci & Technol, Shanghai Frontiers Sci Ctr Optogenet Tech Cell Met, Sch Pharm, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2025

卷号：13

期号：4

起止页码：1395

外文期刊名：JOURNAL OF MATERIALS CHEMISTRY B

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001375908400001)】；

基金：This work was supported by National Key Research and Development Programs (2022YFD1700400 and 2023YFD1700300) and Shanghai Frontiers Science Center of Optogenetic Techniques for Cell Metabolism.

语种：英文

摘要：Intracellular macromolecules have the ability to form membraneless compartments, such as vacuoles and hollow condensates, through liquid-liquid phase separation (LLPS) in order to adapt to changes in their environment. The development of artificial non-homogeneous compartments, such as multiphase hollow or multicavity condensates, has gained significant attention due to their potential to uncover the mechanisms underlying the formation of artificial condensates and biomolecular condensates. However, the complexity of design and construction has hindered progress, particularly in creating dynamic non-homogeneous compartments. In this study, we present a dynamic membraneless compartment using peptide-oligonucleotide conjugates derived from short elastin-like polypeptides (sELP-ONs), which undergo pH-mediated phase transition. Below pH 8.8, the microcompartment exists as microdroplets that transform into non-homogeneous hollow condensates above pH 8.8. Notably, these hollow condensates retain liquid properties and high molecular ordering, and effectively sequester guest molecules with a hollow condensed layer. Furthermore, our sELP-ON microcompartments exhibit a feedback-induced phase transition in response to environmental pH fluctuations generated by complex enzymatic reactions mimicking cellular metabolism, providing a novel dynamic model for creating biomimetic membraneless compartments.