文献类型：专利

英文题名：Culture medium, useful for producing influenza vaccine, comprises e.g. basal metabolism nutrients, nucleic acid composition, inorganic salt compound, pH buffering agent, pH indicator, virus proliferation accelerating agent and additive

作者：FAN L;HUANG D;LIU X;TAN W;ZHAO L

机构：[1]UNIV EAST CHINA SCI & TECHNOLOGY

申请号：CN103045533-A

申请日：2013-01-14

公开日：2013-04-17

语种：英文

收录：DERWENT

摘要：NOVELTY - Culture medium comprises basal metabolism nutrients, nucleic acid composition, vitamins, inorganic salt compound, shear force protection agent, pH buffering agent, pH indicator, virus proliferation accelerating agent and additive, where: the basal metabolism nutrients comprises glucose; the vitamin comprises biotin; the inorganic salt compound comprises copper sulfate; the pH buffering agent comprises sodium bicarbonate; the pH indicator is phenol red; the virus proliferation accelerating agent comprises cholesterol and the additive comprises transferring. USE - The culture medium is useful for producing influenza vaccine (claimed). DETAILED DESCRIPTION - Culture medium comprises basal metabolism nutrients, nucleic acid composition, vitamins, inorganic salt compound, shear force protection agent, pH buffering agent, pH indicator, virus proliferation accelerating agent and additive, where: the basal metabolism nutrients comprises glucose, sodium pyruvate, alanine, arginine, asparagine, aspartic acid, cystine, cysteine, glutamic acid, glutamine, glycine, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tryptophan, tyrosine and valine; the nucleic acid compound comprises hypoxanthine and thymidine; the vitamin comprises biotin, folic acid, nicotinamide, pyridoxine, hydrochloride, thiamine and lipoic acid; the inorganic salt compound comprises copper sulfate, ferric sulfate, ferric nitrate, ferrous sulfate, magnesium sulfate, potassium chloride, sodium chloride, disodium hydrogen phosphate, sodium dihydrogen phosphate and sodium selenite; the shear force protection agent is block type polyether F68, the pH buffering agent comprises sodium bicarbonate and hydroxyethyl piperazine ethyl sulfonic acid; the pH indicator is phenol red; the virus proliferation accelerating agent comprises cholesterol, tocopherol acetate, myristic acid, palmitic acid, palmitoleic acid, stearic acid, magnesium chloride, calcium chloride, sodium butyrate and dimethyl sulfoxide; and the additive comprises transferrin, insulin and soybean hydrolysate.