文献类型：期刊文献

英文题名：Evaluation of the effects of active fractions of chinese medicine formulas on IL-1β, IL-6, and TNF-α release from ANA-1 murine macrophages

作者：Ni, Li-Jun[1];Wang, Nan-Nan[1];Zhang, Li-Guo[1];Guo, Yan-Zi[1];Shi, Wan-Zhong[2]

机构：[1]E China Univ Sci & Technol, Sch Chem & Mol Engn, Post Box 425,130 Mei Long Rd, Shanghai 200237, Peoples R China;[2]Shanghai Univ TCM, Shuguang Hosp, 185 Puan Rd, Shanghai 200021, Peoples R China

年份：2016

卷号：179

起止页码：420

外文期刊名：JOURNAL OF ETHNOPHARMACOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000370884400042)】；

基金：The authors are extremely grateful to Miss Xu Yanjuan for her guidance during the experiment process. We are also extremely grateful to the Shanghai Science and Technology Committee for financially supporting this research (contact No. 13401901100).

语种：英文

外文关键词：Yaotongning formula; Traditional Chinese Medicine; Active fractions; Immune response

摘要：Ethnopharmacological relevance: Yaotongning (YTN) is a traditional Chinese Medicine (TCM) that contains ten component medicinal materials (CMMs) and uses Chinese rice wine as a vehicle to enhance its efficacy. YTN has been used for rheumatoid arthritis (RA) treatment in China for decades and has been reported to have anti-inflammatory and analgesic effects, as well as to strengthen the immune system. Aim of the study: The present work quantitatively evaluated the in vitro effects of active fractions from the ten CMMs that make up YTN and eight additional herbs commonly used in TCM formulas for RA treatment, as well as different combinations of these active fractions, on cellular immune response; the findings were used to determine which active fractions are responsible for promoting an immune response, and to assess whether YTN is superior to other similar formulas and whether YTN can be improved by simplifying its formula from the point of its cellular immunomodulatory activity. Materials and methods: Using the YTN formulation principles and some concepts in combinatorial chemistry, 27 TCM samples were designed by combining some or all of the active fractions of YTN and other eight herbs used for RA treatment. Release of interleukin-1 beta (IL-1p), interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF-alpha) from ANA-1 murine macrophages was measured using an enzyme-linked immunosorbent assay (ELISA). The immunoregulatory effects of the TCM samples were evaluated by comparing their half-effective concentrations (EC50) for stimulating the release of these cytokines. Results: Among the investigated active fractions, the flavonoids from Carthamus tinctorius (Fct), Davallia mariesii (Fdm), and Cinnamomum cassia Twig volatile oils (Vca) from the eight selected herbs effectively promoted IL-1 beta and IL-6 release from ANA-1 cells. Saponins from the YTN CMM Glycyrrhiza uralensis (Sgu) were the most potent promoters of IL-1 beta and TNF-alpha release. The aqueous extract of YTN CMM Eupolyphaga sinensis (Ves) strongly enhanced the release of IL-1(3, IL-6, and TNF-alpha from ANA-1 cells. The EC50 values for stimulating the secretion of IL-1 p, IL-6, and TNF-a could be determined for only six samples. The full YTN formula and the sample containing 50% Glycyrrhiza uralensis saponins, 25% of the mixture of alkaloids, and 25% of the mixture of all flavonoids exhibited good comprehensive cellular immunomodulatory activity. The immunomodulatory activity of the complete YTN formula was better than that of the sample containing all active fractions of YTN without Chinese rice wine (the YTN vehicle). Conclusions: Sgu and Ves are the primary active fractions of YTN involved in stimulating immune responses. The YTN prescription was reasonably effective at promoting cellular immune responses. Chinese rice wine, the YTN vehicle, strengthened the immunoregulatory activity of YTN. The results of this study demonstrate that the YTN recipe could be improved by reducing the number of CMM5 and altering some active fractions without reducing its activity to promote cellular immune responses. (C) 2016 Elsevier Ireland Ltd. All rights reserved.