文献类型：期刊文献

英文题名：Pyomelanin production via heterologous expression of 4-hydroxyphenylpyruvate dioxygenase (HPPD) and construction of HPPD inhibitor screening model

作者：Zhang, Qihao[1];Yang, Xiaohui[1];Lin, Lin[2,3];Wu, Shuhong[3,4];Wang, Ping[5];Wei, Wei[1];Wei, Dongzhi[1]

机构：[1]East China Univ Sci & Technol, Newworld Inst Biotechnol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]Shanghai Univ Med & Hlth Sci, Shanghai Key Lab Mol Imaging, 279 Zhouzhu Rd, Pudong New Area, Shanghai 201318, Peoples R China;[3]Natl Engn Res Ctr Nanotechnol, Res Lab Funct Nanomat, 28 Jiangchuan East Rd, Shanghai 200241, Peoples R China;[4]Shanghai Jiao Tong Univ, Sch Mat Sci & Engn, 800 Dongchuan Rd, Shanghai 200240, Peoples R China;[5]Weigao Shanghai R&D Ctr, 720 Cailun Rd, Pudong New Area, Shanghai 201203, Peoples R China

年份：2023

卷号：135

期号：2

起止页码：93

外文期刊名：JOURNAL OF BIOSCIENCE AND BIOENGINEERING

收录：;EI(收录号：20224913215454);WOS:【SCI-EXPANDED(收录号:WOS:000930752900001)】；

基金：This work was supported by the Shanghai Natural Science Foundation (No. 20ZR1415400), the National Key Research and Development Program of China (2021YFC2100300), the Shanghai outstanding technical leaders plan 19XD1431900, 19XD1431800, the National Natural Science Foundation of China (Grant No. 81830052, 81530053) and the Shanghai Key Laboratory of Molecular Imaging (18DZ2260400). The authors declare no con flict of interest.

语种：英文

外文关键词：  Pyomelanin production; 4-Hydroxyphenylpyruvate dioxygenase; 4-Hydroxyphenylpyruvate dioxygenase inhibitor; Screening model; UV protection]

摘要：Available online 2 December 2022 Melanin has an increasing market demand in cosmetics, food, medicine as well as aerospace due to its unique properties. Heterologous expression of 4-hydroxyphenylpyruvate dioxygenase (HPPD) from the melanin-producing strain Streptomyces fungicidicus NW-EN1 in Escherichia coli shortened the fermentation cycle of melanin. HPPD cata-lyzed 4-hydrophenylpyruvate (HPP) to form homologous acid (HGA) and finally form melanin. The purified melanin had the highest absorption peak at 460 nm. Fourier transform infrared spectroscopy and scanning electron microscope scanning showed that the pigment had universal characteristic peaks. The presence of HGA, a predictor of pyomelanin, was identified by high-performance liquid chromatography analysis. The recombinant E. coli produced 804.4 +/- 5.9 mg/L pyomelanin within 48 h. Metal ions had a great influence on the production of pyomelanin. Pyomelanin was stable in response to light intensity and had a protective effect against bacteria under UV irradiation. Meanwhile, we utilized the chromogenic effect after whole-cell catalysis to reflect the inhibition of the HPPD inhibitors (mesotrione and isoxa-flutole) on HPPD by observing the color change. As a rapid method to test the action of inhibitors, this method is ex-pected to be useful for the development of HPPD-inhibiting herbicides.(c) 2022, The Society for Biotechnology, Japan. All rights reserved.