文献类型：期刊文献

英文题名：Green access to chiral Vince lactam in a buffer-free aqueous system using a newly identified substrate-tolerant (-)-γ-lactamase

作者：Yin, Jin-Gang[1];Gong, Yi[1];Zhang, Xiao-Yan[1];Zheng, Gao-Wei[1];Xu, Jian-He[1]

机构：[1]East China Univ Sci & Technol, Shanghai Collaborat Innovat Ctr Biomfg, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2016

卷号：6

期号：16

起止页码：6305

外文期刊名：CATALYSIS SCIENCE & TECHNOLOGY

收录：;EI(收录号：20163302716301);WOS:【SCI-EXPANDED(收录号:WOS:000381439400015)】；

基金：This work was financially supported by the National Natural Science Foundation of China (no. 21472045, 21506055 & 21536004), the Ministry of Science and Technology, P.R. China (no. 2011AA02A210 & 2012AA022206C) and the Science and Technology Commission of Shanghai Municipality (15JC1400403).

语种：英文

外文关键词：Data mining

摘要：A novel non-heme chloroperoxidase (SvGL) with promiscuous (-)-gamma-lactamase activity towards Vince lactam was identified from Streptomyces viridochromogenes by genome data-mining. SvGL possesses high activity and excellent thermal stability and enantioselectivity. Furthermore, it is able to tolerate extremely high substrate concentrations (4.0 M, 436.5 g L-1). Using the newly discovered (-)-gamma-lactamase as a biocatalyst, an efficient and environmentally benign process for the production of (+)-gamma-lactam was developed. The process allowed an enantioselective resolution of 436.5 g L-1 racemic gamma-lactam with only 0.2 g L-1 lyophilized cell-free extract, affording an extremely high substrate/catalyst ratio of 2183 (g g(-1)), a space-time yield of 458 g L-1 d(-1), and a very low E factor (environmental factor) of 5.7 (kg waste per kg product) even when the process water is included.