文献类型：期刊文献

英文题名：Low oxygen tension favored expansion and hematopoietic reconstitution of CD34+ CD38- cells expanded from human cord blood-derived CD34+ Cells

作者：Wang, Ziyan[1];Du, Zheng[1];Cai, Haibo[1];Ye, Zhaoyang[1];Fan, Jinli[1];Tan, Wen-Song[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2016

卷号：11

期号：7

起止页码：945

外文期刊名：BIOTECHNOLOGY JOURNAL

收录：;EI(收录号：20162802584459);WOS:【SCI-EXPANDED(收录号:WOS:000379786700009)】；

基金：This work was supported by the National Nature Science Foundation of China (20776043), the Key Project of Medicine, Shanghai (074319109) and the Science and Technology Innovation Action Plan of Basic Research, Shanghai (15JC1401402).

语种：英文

外文关键词：CD34(+)CD38(-)cells; Ex vivo expansion; Hematopoietic reconstitution; NOD; SCID mice; Oxygen tension

摘要：Oxygen tension is an important factor that regulates hematopoietic stem cells (HSCs) in both in vivo hematopoietic microenvironment and ex vivo culture system. Although the effect of oxygen tension on ex vivo expansion of HSCs was extensively studied, there were no clear descriptions on physiological function and gene expression analysis of HSCs under different oxygen tensions. In this study, the effects of oxygen tension on ex vivo expansion characteristics of human umbilical cord blood (UCB)-derived CD34(+) cells are evaluated. Moreover, the physiological function of expanded CD34(+) cells was assessed by secondary expansion ability ex vivo and hematopoietic reconstitution ability in vivo. Also, genetic profiling was applied to analyze the expression of genes related to cell function. It was found that low oxygen tension favored expansion of CD34(+)CD38(-) cells. Additionally, CD34(+) cells expanded under low oxygen tension showed better secondary expansion ability and reconstitution ability than those under atmospheric oxygen concentration. Finally, the genetic profiling of CD34(+)CD38(-) cells cultured under low oxygen tension was more akin to freshly isolated cells. These results collectively demonstrate that low oxygen tension was able to better maintain both self-renewal and hematopoietic reconstitution potential and may lay an experimental basis for clinical transplantation of HSCs.