文献类型：期刊文献

英文题名：Discrimination of oligonucleotides of different lengths with a wild-type aerolysin nanopore

作者：Cao, Chan;Ying, Yi-Lun;Hu, Zheng-Li;Liao, Dong-Fang;Tian, He;Long, Yi-Tao[1]

机构：[1]East China Univ Sci & Technol, Key Lab Adv Mat, Shanghai 200237, Peoples R China; East China Univ Sci & Technol, Dept Chem, Shanghai 200237, Peoples R China

年份：2016

卷号：11

期号：8

起止页码：713

外文期刊名：Nature Nanotechnology

收录：;EI(收录号：20193907474002);WOS:【SCI-EXPANDED(收录号:WOS:000381008300016)】；

基金：We thank M. Dal Peraro and F.G. van der Goot for the helpful discussions on the structure of aerolysin. This work was supported by the National Natural Science Foundation of China (grant nos 21327807, 21421004), the 111 Project (grant no. B16017) and National Basic Research Program of China (973 Program) (Grant no. 2013CB733700). Y.-T. L. is supported by the Chang Jiang Scholars Program.

语种：英文

外文关键词：Nanopores - Proteins

摘要：Protein nanopores offer an inexpensive, label-free method of analysing single oligonucleotides. The sensitivity of the approach is largely determined by the characteristics of the pore-forming protein employed, and typically relies on nanopores that have been chemically modified or incorporate molecular motors. Effective, high-resolution discrimination of oligonucleotides using wild-type biological nanopores remains difficult to achieve. Here, we show that a wild-type aerolysin nanopore can resolve individual short oligonucleotides that are 2 to 10 bases long. The sensing capabilities are attributed to the geometry of aerolysin and the electrostatic interactions between the nanopore and the oligonucleotides. We also show that the wild-type aerolysin nanopores can distinguish individual oligonucleotides from mixtures and can monitor the stepwise cleavage of oligonucleotides by exonuclease I.