文献类型：期刊文献

英文题名：Characterization and regulation of the 2,3-butanediol pathway in Serratia marcescens

作者：Rao, Ben[1];Zhang, Liao Yuan[2];Sun, Jian'an[1];Su, Gang[1];Wei, Dongzhi[1];Chu, Ju;Zhu, Jiawen[3];Shen, Yaling[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, New World Inst Biotechnol, Shanghai 200237, Peoples R China;[2]Fujian Agr & Forestry Univ, Sch Life Sci, Fuzhou, Fujian, Peoples R China;[3]E China Univ Sci & Technol, Chem Engn Res Ctr, Shanghai 200237, Peoples R China

年份：2012

卷号：93

期号：5

起止页码：2147

外文期刊名：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

收录：;EI(收录号：20121114848325);WOS:【SCI-EXPANDED(收录号:WOS:000300657900032)】；

基金：We thank Staffan Kjelleberg (The University of New South Wales) for providing S. marcescens MG1. This work was supported by Chinese National Program for High Technology and Development (863 program; no. 2006AA02Z243), Shanghai Leading Academic Discipline Project (project B505) and National Special Fund for State Key Laboratory of Bioreactor Engineering (no. 2060204).

语种：英文

外文关键词：Serratia marcescens; Acetoin; 2,3-Butanediol; Regulation

摘要：Serratia marcescens has been proved to be a potential strain for industrial 2,3-butanediol production for its high yield, productivity, and other advantages. In this study, the genes slaA, slaB, slaC, and slaR were successfully cloned which were further confirmed to be encoding acetolactate decarboxylase, acetolactate synthase, 2,3-butanediol dehydrogenase, and a LysR-like regulator, respectively. Unlike in Klebsiella sp. or Klebsiella pneumonie and Vibrio sp. or Vibrio cholerae, the gene slaC is separated from other genes. Then it showed that two regulators, SwrR and SlaR, are in charge of this process by exerting effect on the transcription of genes slaA and slaB. By contrast, the expression of gene slaC is unaffected by the two regulators. It means that these two regulators affect the production of 2,3-butanediol by regulating the production of acetoin. Based on these findings, we successfully accelerated the 2,3-butanediol production by inactivation of gene swrR. The obtained results and further investigations should lead to a more suitable fermentation strategy and strain improvement which would be applicable to the industrial production of 2,3-butanediol.