文献类型：期刊文献

中文题名：粘质沙雷氏菌产2,3-丁二醇培养基的优化

英文题名：Optimization of fermentation medium for 2,3-butanediol production by Serratia marcescens

作者：李元芳[1];徐勤[2];曹学[1];王学东[1];沈亚领[1];魏东芝[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室鲁华生物技术研究所,上海200237;[2]湖北轻工职业技术学院轻化工系,武汉430070

年份：2007

卷号：37

期号：3

起止页码：24

中文期刊名：工业微生物

外文期刊名：Industrial Microbiology

收录：北大核心:【北大核心2004】；CSCD:【CSCD2011_2012】；

语种：中文

中文关键词：2,3-丁二醇;粘质沙雷氏菌;柠檬酸;培养基;响应面分析

外文关键词：2,3-butanediol; Serratia marcescens ; citric acid; medium; response surface analysis

摘要：研究了各种碳源、氮源、柠檬酸及无机盐对细胞生长与产物形成的影响,通过单因子、正交及中心组合设计响应面分析优化发酵培养基。结果表明在培养基中添加柠檬酸不但可以促进细胞生长与糖耗速度,还可以缩短发酵周期,提高2,3-丁二醇的产量。采用优化后的培养基,2,3-丁二醇的产量由14.03g/L增加到39.27g/L,提高了近3倍。
Effects of several carbon souroes, nitrogen sources, citric add and initial concentration of sucrose on cell growth and 2,3- butanediol production by Serratia marcescens were investigated. The concentrations of medium components were optimized by the methods of one factor, orthogonal experiment and Box-Behnken design, and response surface analysis methods. Moreover, results showed that dtric acid added in medium could improve cell growth and sugar utilization, reduce fermentation time and stimulate the production of 2,3-butanediol. It could accumulate 2,3-butanediol from 14.03 g/L up to 39.27 g/L.