文献类型：期刊文献

英文题名：A new mitochondria-targeted ratiometric fluorescent probe based on diketopyrrolopyrrole for imaging endogenous HOCl in living cells

作者：Xie, Xiaoxu;Wang, Jian;Yan, Yongchao;Zhang, Xiao;Liu, Chenchen;Yang, Ji;Hua, Jianli[1]

机构：[1]East China Univ Sci & Technol, Key Lab Adv Mat, 130 Meilong Rd, Shanghai 200237, Peoples R China; East China Univ Sci & Technol, Sch Chem & Mol Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2018

卷号：143

期号：23

起止页码：5736

外文期刊名：ANALYST

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000450658000010)】；

基金：This work was supported by the National Natural Science Foundation of China (21772040, 21421004, 21372082 and 21572062), the Fundamental Research Funds for the Central Universities (222201717003) and the Programme of Introducing Talents of Discipline to Universities (B16017).

语种：英文

摘要：Hypochlorous acid (HOCl) is a highly effective oxidant produced during phagocytosis and plays an important role in a variety of physiological and pathological processes. In this work, a new ratiometric fluorescent probe (PTZ-TDPP) based on a diketopyrrolopyrrole (DPP) and phenothiazine platform, was firstly designed and synthesized for the selective detection of HOCl. PTZ-TDPP showed an obvious color change from purple to pink along with a distinct fluorescence change from bright red to yellow in response to HOCl. The limit of detection towards HOCl was calculated to be 190 nM. For biological applications, PTZ-TDPP is of great development potential in detecting endogenous HOCl in living RAW 264.7 cells. A co-localization study employing MitoTracker Green showed that PTZ-TDPP was specifically located in the mitochondria of RAW 264.7 cells. Therefore, PTZ-TDPP has great potential for the detection of endogenous HOCl in living cells.