文献类型：期刊文献

中文题名：虫草素高产菌株的筛选及菌丝体中核苷类成分的分析

英文题名：Screening of Cordyceps Strains with High Cordycepin Contents and Analysis of Nucleotides in Mycelia

作者：刘艳芳[1,2];唐庆九[1];杨焱[1];张劲松[1];冯娜[1];周帅[1];蔡令仪[1];高君辉[1];魏东芝[2]

机构：[1]农业部应用真菌资源与利用重点开放实验室上海市农业科学院食用菌研究所,上海201106;[2]华东理工大学生物反应器工程国家重点实验室鲁华生物技术研究所,上海200237

年份：2010

卷号：22

期号：6

起止页码：1061

中文期刊名：天然产物研究与开发

外文期刊名：Natural Product Research and Development

收录：CSTPCD;;CSCD:【CSCD2011_2012】；

基金：2006年国家科技部支撑项目(2006BAD06B08)

语种：中文

中文关键词：深层发酵;虫草素;核苷;胞外液

外文关键词：submerged fermentation; cordycepin; nucleotides; extracellular liquid

摘要：用高效液相色谱对摇瓶培养获得的19个虫草属菌株的菌丝体及胞外液中核苷类成分进行了分析比较。结果表明,菌丝体中含有的核苷种类较多,且不同菌株菌丝体核苷类成分含量差异显著,而胞外液中的核苷主要为虫草素。同时发现虫草素主要分泌到培养基中,胞外液虫草素的含量可比菌丝体中高出40倍。通过筛选,获得一株虫草素高产菌株,培养液中虫草素含量可达366mg/L。
The nucleotides in submerged fermentation preparations of 19 Cordyceps strains were analyzed by using HPLC.The results showed that the nucleotides in mycelia were complicated and their contents in various strains were significant difference.Cordycepin was the main peak in liquid medium,and the yield of cordycepin in liquid was about 40 times higher than it in the mycelium.One strain with high cordycepin content was screened out,and the content of cordycepin in the liquid was up to 366 mg/L.