文献类型：期刊文献

英文题名：A novel fluorescent probe with a large stokes shift for cysteine based on dicyanoisophorone

作者：Hou, Jiaqi;Cai, Pengfei;Wang, Chengyun;Shen, Yongjia[1]

机构：[1]East China Univ Sci & Technol, Key Lab Adv Mat, 130 Meilong Rd, Shanghai 200237, Peoples R China; East China Univ Sci & Technol, Inst Fine Chem, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2018

卷号：59

期号：26

起止页码：2581

外文期刊名：TETRAHEDRON LETTERS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000436221100020)】；

基金：This work was supported by National Natural Science Foundation of China (No. 21576087) and Shanghai Institutes for Biological Sciences.

语种：英文

外文关键词：Fluorescent probe; Dicyanoisophorone; Large stokes shift; Cysteine; Cell imaging

摘要：In this work, a dicyanoisophorone-based turn-on fluorescent probe, DCIP, for highly selective and sensitive detection of cysteine was designed based on nucleophilic substitution mechanism. Moreover, compared with typical cysteine probes, DCIP showed great selectivity and sensitivity for cysteine over other amino acids including the similar structured homocysteine (Hcy) and glutathione (GSH). Further, the detection limit toward cysteine was calculated to be as low as 0.70 mu M. In addition, the utility of DCIP as a bioanalytical molecular tool was demonstrated by fluorescence imaging of biothiols in living cells. (C) 2018 Elsevier Ltd. All rights reserved.