文献类型：期刊文献

英文题名：Phylogenetic diversity of the archaeal community in a continental high-temperature, water-flooded petroleum reservoir

作者：Li, Hui; Yang, Shi-Zhong; Mu, Bo-Zhong

机构：[1]E China Univ Chem Technol, State Key Lab Bioreactors Engn, Shanghai 200237, Peoples R China;[2]E China Univ Chem Technol, Inst Appl Chem, Shanghai 200237, Peoples R China

年份：2007

卷号：55

期号：5

起止页码：382

外文期刊名：CURRENT MICROBIOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000249979500003)】；

语种：英文

摘要：The diversity of an archaeal community was analyzed in the water from a continental high-temperature, long-term water-flooded petroleum reservoir in Huabei Oilfield in China. The archaea were characterized by their 16S rRNA genes. An archaeal 16S rDNA clone library was constructed from the DNA isolated from the formation water, and 237 randomly selected positive clones were clustered in 28 phylotypes by sequencing analyses. Phylogenetic analysis of these sequences indicated that the dominant members of the archaeal phylotypes were affiliated with the order Methanomicrobiales. Totally, the archaeal community was composed of methanogens belonging to four orders: Methanobacteriales, Methanococcales, Methanomicrobiales, and Methanosarcinales. Most of the clones clustered with sequences previously described for methanogens, but there was a difference in the relative distribution of sequences detected here as compared to that of previous studies. Some thermophilic methanogens detected had been previously isolated from a number of high-temperature petroleum reservoirs worldwide; thus, they might exhibit adaptations to the environments and be the common habitants of geothermally heated subsurface environments.