文献类型：期刊文献

英文题名：Stationary phase-dependent accumulation of ectoine is an efficient adaptation strategy in Vibrio anguillarum against cold stress

作者：Ma, Yue[1,2];Wang, Qiyao[1,2,3];Xu, Wensheng[4];Liu, Xiaohong[1,2,3];Gao, Xiating[1];Zhang, Yuanxing[1,2,3]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Shanghai Engn Res Ctr Maricultured Anim Vaccines, Shanghai 200237, Peoples R China;[3]Shanghai Collaborat Innovat Ctr Biomfg, Shanghai 200237, Peoples R China;[4]Beijing Univ Agr, Food Sci & Engn Coll, Beijing 102206, Peoples R China

年份：2017

卷号：205

起止页码：8

外文期刊名：MICROBIOLOGICAL RESEARCH

收录：;EI(收录号：20173504082685);WOS:【SCI-EXPANDED(收录号:WOS:000412962800002)】；

基金：This work was supported by grants from the National Natural Science Foundation of China (No. 31272701), Project of Marine Public Welfare Profession (201405003-2), the Ministry of Agriculture of China (No. CARS-50), and General program of science and technology development project of Beijing Municipal Education Commission (KM201610020015). We thank Miao Guo at the Nuclear Magnetic Resonance Core Facility in ECUST for help with NMR analysis.

语种：英文

外文关键词：Vibrio anguillarum; Compatible solutes; Ectoine; ectABC; proVWX; Cold adaptation

摘要：The capability of cold-adaptation is a prerequisite of microorganisms that survive in an environment with frequent fluctuations in temperature. As a global causative agent of vibriosis in marine fish farming, Vibrio anguillarum can efficiently grow and proliferate under cold-stress conditions, which is 15 degrees C lower than the optimal growth temperatures (25-30 degrees C). Our data showed that V. anguillarum was able to synthesize ectoine de novo and that ectoine was essential for its growth under cold stress. Using H-1 nuclear magnetic resonance spectroscopy and mutants lacking ectABC and proVWX (ectoine synthesis and transporter system genes, respectively), we confirmed that accumulation of this compatible solute occurs strictly at low temperatures and that the expression of ectA and proV is highly activated in the stationary growth phase. However, the synthesis of ectoine was repressed by exogenous choline (precursor of glycine betaine), suggesting that ectoine is an alternative compatible solute as a cold-stress protectant in V. anguillarum. Based on these results, we present possible scenarios of the synthesis and uptake of ectoine, which will facilitate the understanding of the molecular mechanism of V. anguillarum adaptation to cold environments and help improve freezing-dry processes for the V. anguillarum live vaccine.