文献类型：期刊文献

中文题名：Candida krusei的分批培养与补料分批培养生产甘油

英文题名：PRODUCTION OF GLYCEROL IN BATCH AND FED-BATCH CULTIVATION PROCESSES OF CANDIDA KRUSEI

作者：唐军[1];黄良军[1];蔡水洪[2];叶勤[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室,上海200237;[2]华东理工大学化学工程研究所,上海200237

年份：2001

卷号：17

期号：1

起止页码：50

中文期刊名：化学反应工程与工艺

外文期刊名：Chemical Reaction Engineering and Technology

收录：CSTPCD;;Scopus;北大核心:【北大核心2000】；CSCD:【CSCD2011_2012】；

基金：国家九五攻关课题专项 !(96 - C0 3- 0 3- 0 3

语种：中文

中文关键词：克鲁斯假丝酵母;分批培养;补料分批培养;菌体得率系数;甘油;发酵法生产

外文关键词：Candida krusei; batch culture; fed batch culture; cell yield; glycerol

摘要：采用 Candida krusei分批发酵生产甘油 ,因菌体生长阶段形成副产物 ,甘油总得率仅为 0 .377g/ g。生长阶段改用流加培养 ,限制葡萄糖的供应 ,然后补入剩余葡萄糖 ,这样 ,菌体对葡萄糖的得率系数和甘油总得率比分批培养分别提高了 0 .2 5 8g/ g和 0 .0 5 8g/ g,最终甘油浓度提高了 10 g/ L
The glycerol yield in batch cultivation of Candida krusei was only 0.377 g/g due to by-products formation during the cell growth phase. To limit glucose consumption and suppress the formation of by-products, fed-batch cultures were conducted, and glucose was continuously added at low rates during the cell growth phase while the rest of glucose was added at the start of the production phase. The cell yield, glycerol yield, and final glycerol concentration in fed-batch culture were increased by 0.258 g/g, 0.058 g/g, and 10 g/L, respectively, compared with those in the batch culture.