文献类型：期刊文献

英文题名：Modulating and modeling aggregation of cell-seeded microcarriers in stirred culture system for macrotissue engineering

作者：Mei, Yang[1];Luo, Houyong[1];Tang, Qiang[1];Ye, Zhaoyang[1];Zhou, Yan[1];Tan, Wen-Song[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2010

卷号：150

期号：3

起止页码：438

外文期刊名：JOURNAL OF BIOTECHNOLOGY

收录：;EI(收录号：20104613389476);WOS:【SCI-EXPANDED(收录号:WOS:000284984300022)】；

基金：This study was financially supported by National Science Foundation of China (NSFC) (Grant No. 20776044). Financial supports from the Fundamental Research Funds for the Central Universities, the National Special Fund for State Key Laboratory of Bioreactor Engineering (Grant No. 2060204) and Shanghai Natural Science Foundation (Grant No. 10ZR1407100) were also acknowledged.

语种：英文

外文关键词：Microcarriers; Macrotissue engineering; Aggregation behavior; Bioreactor

摘要：A recently developed protocol. "microtissue assembly" holds great promise to address the issue of limited mass transfer within engineered large tissue replacements (macrotissues), wherein small "building blocks" (microtissues) are prepared and then assembled into macrotissues. Previous studies suggested that aggregation behavior of microcarrier-based microtissues were very important for macrotissue engineering. However, a systematic study on the aggregation behavior of microtissues is still missing. In this study, to examine the aggregation behavior of microtissues, effects of key operation parameters in dynamic culture including cell seeding density, microcarrier concentration. L-ascorbic acid 2-phosphate (V-c) and agitating speed were investigated. The aggregation process could be divided into three phases (i.e., lag, growth and stable). Aggregation efficiency (S) was found to be modulated by cell seeding density, microcarrier concentration, addition of V-c and agitating speed. A mathematical model correlating the operation parameters with Sat different phases of aggregation was developed and experimentally proved to be able to predict S with varied operation parameters. In the end, a cylindrical macrotissue (diameter x height: 2.0 cm x 0.8 cm) with fairly good integrity and cellularity and uniform cell distribution was successfully engineered through perfusion assembling microtissues with controlled S under selected culture conditions. Our study showed that aggregation of microtissues could be precisely modulated, which would definitely facilitate engineering macrotissues with high quality. (C) 2010 Elsevier B.V. All rights reserved.