文献类型：期刊文献

英文题名：Engineered microbial sensors: providing a new paradigm for disease detection

作者：Shi, Xuan-Ren[1];Zhang, Yi-Fan[1];Zhou, Ying[1];Zou, Zhen-Ping[1];Ye, Bang-Ce[1]

机构：[1]East China Univ Sci & Technol, Lab Biosyst & Microanal, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2025

卷号：13

期号：45

起止页码：14535

外文期刊名：JOURNAL OF MATERIALS CHEMISTRY B

收录：;EI(收录号：20255219808891);WOS:【SCI-EXPANDED(收录号:WOS:001596072900001)】；

基金：This work was sponsored by the National Key Research and Development Program of China (2024YFA0917100), the National Natural Science Foundation of China (22134003), and China Postdoctoral Science Foundation (2024T170274, 2023M741176).

语种：英文

外文关键词：Cost effectiveness - Diagnosis - Medical applications

摘要：Microorganisms can be programmed into engineered microbial sensors (EMSs) capable of responding to disease biomarkers. Compared to traditional diagnostics, EMSs exhibit superior advantages in cost-effectiveness, environmental adaptability, and non-invasive, in situ diagnostics. These advantages have been further bolstered by recent advances in synthetic biology, which have improved the reliability, accuracy, and safety of EMSs in disease detection, particularly in vivo. In this review, we elucidate the fundamental design principles of EMSs and summarize their research progress across diverse diagnostic scenarios. Furthermore, we evaluate recent advances in EMSs encapsulation strategies, including micro- and macro-encapsulation, and their potential for translation into practical applications. Finally, we outline future perspectives and analyze key challenges in the medical applications of EMSs, including biosafety concerns, signal stability issues, and the absence of standardized clinical evaluation protocols.