文献类型：期刊文献

英文题名：Real-time and high-throughput analysis of mitochondrial metabolic states in living cells using genetically encoded NAD+/NADH sensors

作者：Zhao, Yuzheng[1,2,3];Yang, Yi[1,2,3]

机构：[1]East China Univ Sci & Technol, Synthet Biol & Biotechnol Lab, State Key Lab Bioreactor Engn, Shanghai Collaborat Innovat Ctr Biomfg Technol, 130 Mei Long Rd, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, CAS Ctr Excellence Brain Sci, Optogenet & Synthet Biol Interdisciplinary Res Ct, 130 Mei Long Rd, Shanghai 200237, Peoples R China;[3]East China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab New Drug Design, 130 Mei Long Rd, Shanghai 200237, Peoples R China

年份：2016

卷号：100

起止页码：43

外文期刊名：FREE RADICAL BIOLOGY AND MEDICINE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000387995900005)】；

语种：英文

外文关键词：Mitochondria; Cytosol; Metabolic state; Genetically encoded fluorescent sensor; Imaging; High-throughput; NADH; NAD(+)/NADH ratio

摘要：Mitochondria are central organelles that regulate cellular bioenergetics, biosynthesis, and signaling processes. NADH, a key player in cell metabolism, is often considered as a marker of mitochondrial function. However, traditional methods for NADH measurements are either destructive or unable to distinguish between NADH and NADPH. In contrast to traditional methods, genetically encoded NADH sensors can be used for the real-time tracking and quantitative measurement of subcellular NADH levels in living cells. Therefore, these sensors provide innovative tools and address the limitations of current techniques. We herein summarize the properties of different types of recently developed NADH biosensors, discuss their advantages and disadvantages, and focus on the high-throughput analysis of mitochondrial function by using highly responsive NAD(+)/NADH sensors. (C) 2016 Elsevier B.V. All rights reserved.