文献类型：期刊文献

英文题名：Characteristics of oxidative stress and antioxidant defenses by a mixed culture of acidophilic bacteria in response to Co2+ exposure

作者：Wu, Weijin[1];Li, Xiyan[1];Zhang, Xu[1];Gu, Tingyue[2];Qiu, Yongqiu[1];Zhu, Minglong[1];Tan, Wensong[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Ohio Univ, Dept Chem & Biomol Engn, Athens, OH 45701 USA

年份：2020

卷号：24

期号：4

起止页码：485

外文期刊名：EXTREMOPHILES

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000528156600001)】；

基金：This study was supported by the Open Project Funding of the State Key Laboratory of Bioreactor Engineering of China, the National High Technology Research and Development Program of China (nos. 2007AA060904 and 2012AA061503) and the National Natural Science Foundation of China (NSFC21878083).

语种：英文

外文关键词：Bioleaching; Cobalt; ROS; Mixed-culture; Acidophilic bacteria; Antioxidant

摘要：During bioleaching of Cobalt from waste lithium-ion batteries, the biooxidation activity of acidophilic bacteria is inhibited by a high concentration of Co ion in the liquid phase. However, the mechanism for Co2+ toxicity to acidophilic bacteria has not been fully elucidated. In this study, the effects of Co2+ concentration on the biooxidation activity for Fe2+, intracellular reactive oxygen species (ROS) level and antioxidant defense systems in a mixed-culture of acidophilic bacteria (MCAB) were investigated. The results showed that the biooxidation activity of the MCAB was inhibited by Co2+. Furthermore, it was indicated that the intracellular ROS contents of the MCAB under conditions of 0.4 M and 0.6 M Co2+ were 2.60 and 3.34 times higher than that under the condition of 0 M Co2+. The increase in intracellular malondialdehyde content indicated that the oxidative damage was induced by Co2+. Moreover, the antioxidant systems in MCAB were affected by Co2+. It was observed that the Co2+ exposure increased the catalase and glutathione peroxidase activities while reducing the superoxide dismutase activity and the intracellular glutathione (GSH) content. It was found that an exogenous GSH supplementation eliminated excess intracellular ROS and improved the biooxidation activity of the MCAB.