文献类型：期刊文献

英文题名：Regulation of porcine circovirus type 2-like particles expressed in baculovirus expression system

作者：Li, Yingjun[1]; Yi, Xiaoping[1]; Zhuang, Yingping[1]; Chu, Ju[1]

机构：[1] State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Rd., Shanghai, 200237, China

年份：2016

卷号：3

期号：1

外文期刊名：Bioresources and Bioprocessing

收录：EI(收录号：20224513087719)

语种：英文

外文关键词：Animals - Transcription - Viruses

摘要：Background: Porcine circovirus type 2 (PCV2) virus-like particles (VLPs) are an effective vaccine against post-weaning multisystemic wasting syndrome. Burst sequence (BS) and the chicken 5′-HS4 β-globin (HS4) insulator were used to improve the production of PCV2 VLPs in insect cells. Results: BS was used to modify the polyhedron (polh) promoter, and the chicken HS4 insulator was inserted downstream of the target gene expression cassette. This enhanced effect was detected by EGFP, a generally used protein as reporter. The transcription-level analysis demonstrated that the double BS and the chicken HS4 insulator improved the transcription levels of the target genes. At the same time, the two regulatory elements also improved the PCV2 VLPs’ expression. PCV2 VLPs’ expression levels increased by 14 and 24% at 72h p.i. Further research observed an additive effect when the double BS and the chicken HS4 insulator simultaneously acted on the expression of PCV2 VLPs, with the PCV2 VLPs’ expression level increasing by 35% at 72h p.i. Conclusions: BS and HS4 insulator can be used to improve the production of PCV2 VLPs in insect cells.[Figure not available: see fulltext.]. ? 2016, The Author(s).