文献类型：期刊文献

英文题名：De novo biosynthesis of 2′-fucosyllactose in engineered Pichia pastoris

作者：Qian, Difan[1];Zhang, Chunyue[1,3];Deng, Chen[1,3];Zhou, Mian[1];Fan, Liqiang[1,3];Zhao, Liming[1,2,3]

机构：[1]East China Univ Sci & Technol, Sch Biotechnol, State Key Lab Bioreactor Engn, Meilong Rd 130, Shanghai 200237, Peoples R China;[2]Shanghai Changzheng Hosp, Organ Transplant Ctr, Shanghai 200003, Peoples R China;[3]Shanghai Collaborat Innovat Ctr Biomfg Technol SCI, Shanghai 200237, Peoples R China

年份：2023

卷号：45

期号：4

起止页码：521

外文期刊名：BIOTECHNOLOGY LETTERS

收录：;EI(收录号：20230813600155);WOS:【SCI-EXPANDED(收录号:WOS:000935556400001)】；

基金：AcknowledgementsThis research was supported by the National Key R&D Program of China (Grant Number 2019YFD0901800), Shanghai Post-doctoral Excellence Program (2020132), Natural Science Foundation of Shanghai (22ZR1418200) and the 111 Project (B18022).

语种：英文

外文关键词：Biosynthesis; 2 '-Fucosyllactose; Human milk oligosaccharides; Pichia pastoris

摘要：Purpose Pichia pastoris is well known for its ability to produce short and low-immunogenic humanized glycosyl chains onto recombinant glycoproteins, it was thus speculated to be applicable to synthesize oligosaccharides. In this study, generally recognized as safe (GRAS) microorganism Pichia pastoris GS115 was tested for its potential to be used as a new synthetic chassis to produce the most abundant human milk oligosaccharide 2 '-fucosyllactose (2 '-FL).Methods To enable the de novo synthesis of 2 '-FL, lactose transporter lac12, two enzymes of gmd, gmer, and fucosyltransferases futC were integrated into the genome of P. pastoris, under the control of constitutive PGAP promoter. Results The resulting recombinant yeasts yielded up to 0.276 g/L through culture optimization in a 5 L bioreactor.Conclusion To our knowledge, this is the first report of 2 '-FL production in engineered Pichia pastoris. This work is a good starting point to produce 2 '-FL using Pichia pastoris as a viable chassis.