文献类型：期刊文献

英文题名：Regulation of Vibrio alginolyticus virulence by the LuxS quorum-sensing system

作者：Ye, J.[1];Ma, Y.[1];Liu, Q.[1];Zhao, D. L.[1];Wang, Q. Y.[1];Zhang, Y. X.[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2008

卷号：31

期号：3

起止页码：161

外文期刊名：JOURNAL OF FISH DISEASES

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000252930300001)】；

语种：英文

外文关键词：autoinducer AI-2; luxS; quorum sensing; Vibrio alginolyticus; virulence

摘要：Quorum sensing (QS) is a bacterial intercommunication system that controls the expression of multiple genes in response to population density. The LuxS QS system regulates the expression of several virulence factors in a wide variety of pathogenic bacteria. LuxS has been characterized to be responsible for producing a type of autoinducer, AI-2, which stimulates the expression of the luciferase operon in Vibrio harveyi. Vibrio alginolyticus is established as an opportunistic pathogen of several marine animals, and its LuxS QS system remains undefined. To investigate the pathogenic role of luxS in V. alginolyticus, the luxS mutants of both the standard strain ATCC 33787 and a fish-clinical isolate MVP01, named MYJS and MYJM, respectively, were constructed. The mutation resulted in reduced lethality to Pagrus major. Intraperitoneal LD50 of MYJS and MYJM increased by 15- and 93-fold, respectively. The two luxS mutants exhibited a lower growth rate and defective flagellar biosynthesis. They also showed a significant decrease in protease production and an increase in both extracellular polysaccharide production and biofilm development. The results suggest that the LuxS QS system plays an important role in regulating the expression of virulence factors in V. alginolyticus.