文献类型：期刊文献

英文题名：Genomic characteristics of a novel strain Lactiplantibacillus plantarum X7021 isolated from the brine of stinky tofu for the application in food fermentation

作者：Liu, Guang[1];Liu, Yafan[1];Ro, Kum-Song[1];Du, Lei[1];Tang, Ya-Jie[2];Zhao, Li[1];Xie, Jingli[1,3];Wei, Dongzhi[1,3]

机构：[1]East China Univ Sci & Technol, Inst New World Biotechnol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Shandong Univ, State Key Lab Microbial Technol, Qingdao 266237, Peoples R China;[3]Shanghai Collaborat Innovat Ctr Biomfg SCICB, Shanghai 200237, Peoples R China

年份：2022

卷号：156

外文期刊名：LWT-FOOD SCIENCE AND TECHNOLOGY

收录：;EI(收录号：20220211448895);WOS:【SCI-EXPANDED(收录号:WOS:000766110000006)】；

基金：This work was supported by National Key Research and Development Program of China (No. 2020YFA0907800), National Natural Science Foundation of China (Grant No. 31801489), Natural Science Foundation of Shanghai (No.21ZR1416200), and State Key Laboratory of Microbial Technology Open Projects Fund (Project No. 62450004042010), P. R. China.

语种：英文

外文关键词：Lactiplantibacillus plantarum; Comparative genomic analysis; beta-glucosidase; Purine metabolism; Nitrite degradation

摘要：Lactiplantibacillus plantarum X7021 is a novel strain originated from the brine of stinky tofu. In order to investigate the application potential of the strain in food industry, the genomic characteristics as well as some metabolic features were studied using comparison genomics and transcriptional assay. The complete genome of X7021includes a 3,407,054 bp chromosome with 3259 protein coding sequences, and 6 plasmids. L. plantarum strains, WCFS1, JDM1, and ST-III were selected for comparative genomic analysis. Results revealed that X7021 is safe for the application in foods with less antibiotic resistance and virulence genes. The strain has 25 complete phosphotransferase system transporters and strong proteolytic system for its adaptability to various foodstuffs. The strain containes a complete purine metabolic pathway incorporating 4 permeases, resulting in the reductions of 38.9% guanine, 26.1% hypoxanthine and 70.2% xanthine during the soymilk fermentation. Soy isoflavone glycosides could be converted to corresponding aglycones by two beta-glucosidases. The strain could grow in 700 mg/L nitrite and entirely degrade 100 mg/L nitrite during 24 h based on highly active nitrite transporter and nitrite reductase. Accordingly, the results suggest that L. plantarum X7021 is a prospect starter candidate for the fermentation of plant foodstuffs.