文献类型：期刊文献

英文题名：Quantitative analysis of the growth of individual Bacillus coagulans cells by microdroplet technology

作者：Zhu, Xudong[1];Shi, Xiang[1];Chu, Ju[1];Ye, Bangce[1];Zuo, Peng[1];Wang, Yonghong[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Coll Biotechnol, POB 329,130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2018

卷号：5

期号：1

外文期刊名：BIORESOURCES AND BIOPROCESSING

收录：;EI(收录号：20224513086018);WOS:【SCI-EXPANDED(收录号:WOS:000461106800045)】；

基金：This work was supported by the National Science Foundation for Young Scientists of China (31700038) and the National Key Research and Development Program (2017YFB0309302).

语种：英文

外文关键词：Bacillus coagulans; Microdroplets; Morphological heterogeneity; Single cell

摘要：Background: Cellular physiological responses, which are often obscured by inferences from population-level data, are of great importance in cell biology. Microfluidics has emerged as an important tool for biological research on a small scale, reaching even the single-cell level. Results: In this work, a flow-focusing microdroplet generator was developed to produce monodisperse microdroplets with high stability. Individual B. coagulans cells were encapsulated in the microdroplets and cultured offline. The specific growth rate of B. coagulans at the single-cell level was analyzed, and the growth of B. coagulans in the droplets showed good consistency with that in flasks, with a correlation coefficient of 0.98. The morphological heterogeneity and its potential relationship with the production of lactic acid by B. coagulans were evaluated using a microscopic imaging method. Conclusion: We have demonstrated a single-cell monitoring methodology based on microdroplets. This approach has great potential for studying a range of behavioral and physiological features of bacteria at the single-cell level.