文献类型：期刊文献

英文题名：Swift Covalent Gelation Coupled with Robust Wet Adhesive Powder: A Novel Approach for Acute Massive Hemorrhage Control in Dynamic and High-Pressure Wound Environments

作者：Chen, Kangli[1];Wang, Kun[1];Pan, Yanjun[2];Zhang, Yi[1];Zhang, Jiajun[1];Ji, Shizhao[3];Yin, Meng[2];Liu, Changsheng[1];Qu, Xue[1,4,5]

机构：[1]East China Univ Sci & Technol, Frontiers Sci Ctr Materiobiol & Dynam Chem, Sch Mat Sci & Engn, Key Lab Ultrafine Mat,Minist Educ, Shanghai 200237, Peoples R China;[2]Shanghai Jiao Tong Univ, Shanghai Childrens Med Ctr, Sch Med, Dept Pediat Cardiothorac Surg, 1678 Dong Fang Rd, Shanghai 200127, Peoples R China;[3]Second Mil Med Univ, Changhai Hosp, Inst Burns, Dept Burn Surg, Shanghai 200433, Peoples R China;[4]Shanghai Univ, Wenzhou Inst, Wenzhou 325000, Peoples R China;[5]Shanghai Frontier Sci Ctr Optogenet Tech Cell Meta, Shanghai 200237, Peoples R China

年份：2024

卷号：20

期号：33

外文期刊名：SMALL

收录：;EI(收录号：20241715957057);WOS:【SCI-EXPANDED(收录号:WOS:001205652300001)】；

基金：K.C., K.W. and Y.P. contributed equally to this work. This research was supported by the National Key Research and Development Program (2021YFB3800800), the National Natural Science Foundation of China (31922041, 11932012, 32171341, 82202334), the 111 Project (B14018), Excellence Project of Shanghai Municipal Health Commission (20234Z0003), the Science and Technology Innovation Project and Excellent Academic Leader Project of Shanghai Science and Technology Committee (21S31901500, 21XD1421100) were acknowledged.

语种：英文

外文关键词：dynamic and high-pressure wound; emergency hemostasis; gelation time; self-gelling powder; wet adhesion

摘要：The quest for efficient hemostatic agents in emergency medicine is critical, particularly for managing massive hemorrhages in dynamic and high-pressure wound environments. Traditional self-gelling powders, while beneficial due to their ease of application and rapid action, fall short in such challenging conditions. To bridge this gap, the research introduces a novel self-gelling powder that combines ultrafast covalent gelation and robust wet adhesion, presenting a significant advancement in acute hemorrhage control. This ternary system comprises epsilon-polylysine (epsilon-PLL) and 4-arm polyethylene glycol succinyl succinate (4-arm-PEG-NHS) forming the hydrogel framework. Na2HPO4 functions as the "H+ sucker" to expedite the amidation reaction, slashing gelation time to under 10 s, crucial for immediate blood loss restriction. Moreover, PEG chains' hydrophilicity facilitates efficient absorption of interfacial blood, increasing the generated hydrogel's cross-linking density and strengthens its tissue bonding, thereby resulting in excellent mechanical and wet adhesion properties. In vitro experiments reveal the optimized formulation's exceptional tissue compliance, procoagulant activity, biocompatibility and antibacterial efficacy. In porcine models of heart injuries and arterial punctures, it outperforms commercial hemostatic agent Celox, confirming its rapid and effective hemostasis. Conclusively, this study presents a transformative approach to hemostasis, offering a reliable and potent solution for the emergency management of massive hemorrhage. This study presents a self-gelling powder comprising Na2HPO4 as the "H+ sucker" with epsilon-PLL and 4-arm-PEG-NHS as the hydrogel skeleton. Na2HPO4/epsilon-PLL/PEG demonstrates ultrafast self-gelation and robust wet adhesion. Applied to the dynamic and high-pressure wounds, the powder rapidly absorbs interfacial blood and undergoes an ultrafast powder-hydrogel transition that creates robust physical sealing while aggregating blood cells to accelerate coagulation. image