文献类型：期刊文献

英文题名：Metabolic engineering of Escherichia coli carrying the hybrid acetone-biosynthesis pathway for efficient acetone biosynthesis from acetate

作者：Yang, Hao[1];Huang, Bing[1];Lai, Ningyu[1];Gu, Yang[4];Li, Zhimin[1,2];Ye, Qin[1];Wu, Hui[1,2,3]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]Shanghai Collaborat Innovat Ctr Biomfg Technol, 130 Meilong Rd, Shanghai 200237, Peoples R China;[3]China Natl Light Ind Council, Key Lab Biobased Mat Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[4]Chinese Acad Sci, Shanghai Inst Plant Physiol & Ecol, CAS Ctr Excellence Mol Plant Sci, Key Lab Synthet Biol, Shanghai 200032, Peoples R China

年份：2019

卷号：18

外文期刊名：MICROBIAL CELL FACTORIES

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000455650700001)】；

基金：This study was supported by the National Natural Science Foundation of China (Grant No. 21776083), the Fok Ying-Tong Education Foundation, China (Grant No. 161017), the National Key Research and Development Program of China (Grant No. 2017YFB0309302), the Science and Technology Commission of Shanghai Municipality (Grant No. 17JC1404800), the Fundamental Research Funds for the Central Universities (Grant No. 22221818014). Partially supported by Open Funding Project of the State Key Laboratory of Bioreactor Engineering.

语种：英文

外文关键词：Acetate; Acetone; Metabolic engineering; Escherichia coli; Gas-stripping; Resting cell

摘要：BackgroundThe shortage of food based feedstocks has been one of the stumbling blocks in industrial biomanufacturing. The acetone bioproduction from the traditional acetone-butanol-ethanol fermentation is limited by the non-specificity of products and competitive utilization of food-based substrates. Using genetically modified Escherichia coli to produce acetone as sole product from the cost-effective non-food based substrates showed great potential to overcome these problems.ResultsA novel acetone biosynthetic pathway were constructed based on genes from Clostridium acetobutylicum (thlA encoding for thiolase, adc encoding for acetoacetate decarboxylase, ctfAB encoding for coenzyme A transferase) and Escherichia coli MG1655 (atoB encoding acetyl-CoA acetyltransferase, atoDA encoding for acetyl-CoA: acetoacetyl-CoA transferase subunit and ). Among these constructs, one recombinant MG1655 derivative containing the hybrid pathway consisting of thlA, atoDA, and adc, produced the highest level of acetone from acetate. Reducing the gluconeogenesis pathway had little effect on acetone production, while blocking the TCA cycle by knocking out the icdA gene enhanced the yield of acetone significantly. As a result, acetone concentration increased up to 113.18mM in 24h by the resting cell culture coupling with gas-stripping methods.ConclusionsAn engineered E. coli strain with optimized hybrid acetone biosynthetic pathway can utilize acetate as substrate efficiently to synthesize acetone without other non-gas byproducts. It provides a potential method for industrial biomanufacturing of acetone by engineered E. coli strains from non-food based substrate.