文献类型：期刊文献

英文题名：A Ratiometric Fluorescent Probe for Monitoring Leucine Aminopeptidase in Living Cells and Zebrafish Model

作者：Zhou, Zhe[1,2];Wang, Feiyi[1,2];Yang, Guichun[1,2];Lu, Cuifen[1,2];Nie, Junqi[1,2];Chen, Zuxing[1,2];Ren, Jun[1,2];Sun, Qi[3,4];Zhao, Chunchang[5,6];Zhu, Wei-Hong[5,6]

机构：[1]Hubei Univ, Minist Educ, Key Lab Synth & Applicat Organ Funct Mol, Wuhan 430062, Hubei, Peoples R China;[2]Hubei Univ, Hubei Collaborat Innovat Ctr Adv Organ Chem Mat, Wuhan 430062, Hubei, Peoples R China;[3]Wuhan Inst Technol, Minist Educ, Key Lab Green Chem Proc, Wuhan 430205, Hubei, Peoples R China;[4]Wuhan Inst Technol, Sch Chem & Environm Engn, Wuhan 430205, Hubei, Peoples R China;[5]East China Univ Sci & Technol, Key Lab Adv Mat, Shanghai 200237, Peoples R China;[6]East China Univ Sci & Technol, Inst Fine Chem, Shanghai 200237, Peoples R China

年份：2017

卷号：89

期号：21

起止页码：11576

外文期刊名：ANALYTICAL CHEMISTRY

收录：;EI(收录号：20191206672710);WOS:【SCI-EXPANDED(收录号:WOS:000414887000059)】；

基金：We gratefully acknowledge the financial support by the National Science Foundation of China (21676075).

语种：英文

外文关键词：Fluorescence - Probes - Diagnosis - Lanthanum compounds - Peptides - Tumors - Amino acids - Cytology - Diseases - Fluorescence spectroscopy

摘要：Leucine aminopeptidase (LAP) is an important cancer-related biomarker, which shows significant over expression in malignant tumor cells like liver cancer. Developing an effective method to monitor LAP in tumor cells holds great potential for cancer diagnosis, treatment, and management. In this work, we report a novel BODIPY-based fluorescent probe (BODIPY-C-Leu) capable of monitoring LAP in vitro and in vivo in both ratiometric and turn-on model. BODIPY-C-Leu contains an asymmetrical BODIPY dye for fluorescent signaling and a dipeptide (Cys-Leu) as the triggered moiety. Activation occurs by cleavage of the amide bond in dipeptides and subsequently an intramolecular S -> N conversion to convert sulfur-substituted BODIPY to amino-substituted BODIPY, resulting in a dramatic fluorescence variation to realize the detection of LAP. Furthermore, we have successfully employed BODIPY-C-Leu to monitor LAP activity in different cancer cells, indicating that HeLa cells have a higher level of LAP activity than A549 cells. Importantly, we demonstrated the capability of the probe for real-time monitoring the drug-induced LAP level changes in zebrafish.